Camacho, ErwinPaternina Gómez, MargaretBlanco Tuirán, Pedro J.Osorio, Jorge E.Aliota, Matthew T.2019-09-272019-09-272019-09-27Camacho, E., Paternina, M., Blanco, P.J., Osorio, J.E. & Aliota, M.T. ( 2016). Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia. Emerging Infectious Diseases. 22(5), 927 – 929.1080-6040https://repositorio.unisucre.edu.co/handle/001/804Artículo digitalDuring October–November 2015, a total of 22 patients received a presumptive diagnosis of an acute viral illness by emergency department physicians at the Centro de Diagnostico Medico-Universidad de Sucre in Sincelejo. The patients began treatment for a dengue-like illness, and blood samples were obtained for diagnosis. The samples were analyzed at the Universidad de Sucre by using reverse transcription PCR (RT-PCR) to detect DENV, CHIKV, or Zika virus. Viral RNA was extracted from the serum samples by using the ZR Viral RNA Kit (Zymo Research, Irvine, CA, USA); reverse transcription was performed by using the Protoscript First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). Amplicons were produced by using the OneTaq Quick-Load 2X Master Mix (New England Biolabs) with primers specific to DENV (7), CHIKV (forward: 5′-CGCCAACATTCTGCTTACAC-3′; reverse: 5′-AGGATGCCGGTCATTTGAT-3′), and Zika virus. Tapplication/pdfengDerechos Reservados - Universidad de Sucre, 2019Artículo de revistainfo:eu-repo/semantics/openAccesshttp://dx.doi.org/10.3201/eid2205.160023Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)AutochthonousZikaVirusInfection