TY - NEWS
TI - Caracteres moleculares para la determinación taxonómica de tres especies de Lutzomyia (Diptera: Psychodidae), vectores potenciales de Leishmania presentes en el valle de Aburrá, Colombia.
AU - Pérez Doria, Alveiro
AU - Bejarano, Eduar Elías
AU - Sierra, Diana
AU - Vélez, Iván Darío
AB - To date, 143 species of Lutzomyia França are recorded in Colombia, but less than 7% is incriminated in the transmission of Leishmania spp. Alternative taxonomic characters are necessary to correctly identify the particular sand fl y fauna in each Colombian region, and the separation of morphologically similar vector and non-vector species. In order to detect useful molecular characters for the taxonomic determination of three potential vectors of Leishmania present in the Valle de Aburrá, Colombia, the present work sequenced the 3’ end of the mitochondrial cytochrome b gene in Lutzomyia hartmanni (Fairchild and Hertig), L. columbiana (Ristorcelli and Van Ty), and L. tihuiliensis Le Pont, Torrez-Espejo and Dujardin. Polymorphic sites, pairwise genetic distances (p), and entropy were determined from the multiple alignment of the nucleotide sequences. Numbers of silent and non silent substitutions were calculated from the amino acid sequences deduced from the nucleotide sequences of the gene. In the multiple alignment of the cytochrome b nucleotide sequences from Lutzomyia hartmanni, L. columbiana and L. tihuiliensis, 83 polymorphic sites were detected. A total of 18 amino acid replacements were found in the partial nucleotide sequences of the protein. Genetic distances varied from 0,137 between L. tihuiliensis and L. columbiana, to 0,215 among L. columbiana and L. hartmanni. Nucleotide and amino acid sequence polymorphisms within the cytochrome b gene and protein, respectively, constitute molecular characters potentially useful for the taxonomic determination of these sand fl y species.
DA - 2019-10-18
KW - Psychodidae
KW - Lutzomyia
KW - Verrucarum
KW - Helcocyrtomyia
KW - Citocromo b
KW - Colombia
PB - Buenos aires, Argentina: Revista de la  Sociedad Entomológica Argentina, 2008.
UR - https://repositorio.unisucre.edu.co/handle/001/878
ER -