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DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites.
dc.contributor.author | Vivero, Rafael José | spa |
dc.contributor.author | Bejarano, Eduar Elías | spa |
dc.contributor.author | Estrada, Luis Gregorio | spa |
dc.contributor.author | Flórez, Fernando | spa |
dc.contributor.author | Uribe Soto, Sandra | spa |
dc.contributor.author | Yamileth Edgar | spa |
dc.contributor.author | Estrada, Luis Gregorio | spa |
dc.contributor.author | Ortega Gómez, Edgar | spa |
dc.contributor.author | Torres Gutiérrez, Carolina | spa |
dc.contributor.author | Muskus López, Carlos | spa |
dc.coverage.spatial | Colombia | spa |
dc.date.accessioned | 2019-09-25T16:28:41Z | spa |
dc.date.available | 2019-09-25T16:28:41Z | spa |
dc.date.issued | 2019-09-25 | spa |
dc.identifier.citation | Vivero, R.J., Bejarano, E.E., Estrada, L.G., Flórez, F., Ortega, E., Aparicio, Y., Torres, C., Uribe, S. & Muskus, C. (2017). DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. Revista zootaxa. 4277(2), 228-236. | spa |
dc.identifier.issn | 1175-5334 | spa |
dc.identifier.uri | https://repositorio.unisucre.edu.co/handle/001/796 | spa |
dc.description | Artículo digital | spa |
dc.description.abstract | Although phlebotomine sand flies breeding sites have been identified and recorded by several studies, the microhabitats exploited by these insects remain little-known and hard to find. In this context, the difficulty of finding immature stages, and the limited number of taxonomic studies to identify immature stages of phlebotomine sand flies, are considered the major obstacles when attempting a complete inventory of Lutzomyia species. The objective of this study is to validate Cytochrome Oxidase I (Barcode region) as a marker for the identification of immature stages of Lutzomyia species recovered from natural breeding sites in Colombia. Among 142 collected sand flies, 18 immature individuals that did not complete their life cycle were identified to species level through sequencing of the COI gene. Values of K2P genetic distance between 0.002–0.031 allowed the identification of larvae at species level. The bootstrap support values (96%) in the Neighbor-Joining dendrogram were consistent for the majority of the established MOTUS of Lutzomyia atroclavata, Lutzomyia micropyga, Lutzomyia serrana, Lutzomyia cayennensis, Lutzomyia rangeliana, Lutzomyia shannoni and some species of the genus Brumptomyia. The COI gene is validated as a marker for the identification of immature stages of the genus Lutzomyia. | eng |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | eng | spa |
dc.publisher | Auckland, Nueva Zelanda: Revista Zootaxa, 2017. | spa |
dc.relation.ispartof | Articulo de revista | spa |
dc.rights | Derechos Reservados - Universidad de Sucre, 2019 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | spa |
dc.source | https://read.qxmd.com/keyword/208412/6 | spa |
dc.title | DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. | spa |
dc.type | Artículo de revista | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.type.driver | info:eu-repo/semantics/article | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | spa |
dc.identifier.doi | https://doi.org/10.11646/zootaxa.4277.2.3 | spa |
dc.relation.references | Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSIBLAST: A new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402. https://doi.org/10.1093/nar/25.17.3389. | spa |
dc.relation.references | Arrivillaga, J.C., Navarro, J.C. & Feliciangeli, D. (1999) Morfología y quetotaxia del tagma cefálico larval de Lutzomyia França, 1924 (Diptera: Psychodidae): Proposición de un sistema de nomenclatura. Boletín de Entomología Venezolana, 14, 1–13. | spa |
dc.relation.references | Azpurua, J., De La Cruz, D., Valderrama, A. & Windsor, D. (2010) Lutzomyia sand fly diversity and rates of infection by Wolbachia and an exotic Leishmania species on Barro Colorado Island, Panamá. Plos Neglected Tropical Diseases, 4, e627. https://doi.org/10.1371/journal.pntd.0000627. | spa |
dc.relation.references | Bates, P.A., Depaquit, J., Galati, E.A., Kamhawi, S., Maroli, M., McDowell, M.A., Picado, A., Ready, P.D., Salomón, O.D., Shaw, J.J., Traub-Cseko, Y. & Warburg, A. (2015) Recent advances in phlebotomine sand fly research related to leishmaniasis control. Parasites & Vectors, 8, 131. https://doi.org/10.1186/s13071-015-0712-x. | spa |
dc.relation.references | Blouin, M.S., Yowell, C.A., Courtney, C.H. & Dame, J. (1998) Substitution bias, rapid saturation, and use of mtDNA for nematode systematics. Molecular Biology and Evolution, 15, 1719–1727. https://doi.org/10.1093/oxfordjournals.molbev.a025898. | spa |
dc.rights.creativecommons | Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) | spa |
dc.subject.proposal | Brumptomyia | spa |
dc.subject.proposal | Lutzomyia | spa |
dc.subject.proposal | Colombia | spa |
dc.subject.proposal | Cytochrome Oxidasel I | spa |
dc.subject.proposal | Mitochondrial DNA | spa |
dc.type.content | Text | spa |
dc.type.redcol | http://purl.org/coar/resource_type/c_2df8fbb1 | spa |
oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |