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In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.
dc.contributor.author | Tournu, Heléne | spa |
dc.contributor.author | Luna Tapia, Arturo | spa |
dc.contributor.author | Peters, Brian M. | spa |
dc.contributor.author | Palmer, Glen E. | spa |
dc.coverage.spatial | Colombia | spa |
dc.date.accessioned | 2019-09-24T21:38:38Z | spa |
dc.date.available | 2019-09-24T21:38:38Z | spa |
dc.date.issued | 2019-09-24 | spa |
dc.identifier.citation | Tournu, H., Luna, A., Peters, B.M. & Palmer, G.E. (2017). In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. Revista mSphere . 2(4). | spa |
dc.identifier.issn | 1098-6596 | spa |
dc.identifier.uri | https://repositorio.unisucre.edu.co/handle/001/791 | spa |
dc.description | Artículo digital. | spa |
dc.description.abstract | Environmental or chemically induced stresses often trigger physiological responses that regulate intracellular pH. As such, the capacity to detect pH changes in real time and within live cells is of fundamental importance to essentially all aspects of biology. In this respect, pHluorin, a pH-sensitive variant of green fluorescent protein, has provided an invaluable tool to detect such responses. Here, we report the adaptation of pHluorin2 (PHL2), a substantially brighter variant of pHluorin, for use with the human fungal pathogen Candida albicans. As well as a cytoplasmic PHL2 indicator, we describe a version that specifically localizes within the fungal vacuole, an acidified subcellular compartment with important functions in nutrient storage and pH homeostasis. In addition, by means of a glycophosphatidylinositolanchored PHL2-fusion protein, we generated a cell surface pH sensor. | eng |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | eng | spa |
dc.publisher | Estados Unidos: mSphere, 2017. | spa |
dc.relation.ispartof | Articulo de revista | spa |
dc.rights | Derechos Reservados - Universidad de Sucre, 2019 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | spa |
dc.source | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497024/ | spa |
dc.title | In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. | spa |
dc.type | Artículo de revista | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.type.driver | info:eu-repo/semantics/article | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | spa |
dc.identifier.doi | 10.1128/mSphere.00276-17 | spa |
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dc.relation.references | Bencina M. 2013. Illumination of the spatial order of intracellular pH by genetically encoded pH-sensitive sensors. Sensors 13:16736 –16758. https://doi.org/10.3390/s131216736. | spa |
dc.relation.references | Liu NN, Köhler JR. 2016. Antagonism of fluconazole and a proton pump inhibitor against Candida albicans. Antimicrob Agents Chemother 60: 1145–1147. https://doi.org/10.1128/AAC.02043-15. | spa |
dc.relation.references | Prosser DC, Whitworth K, Wendland B. 2010. Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras. Traffic 11:1141–1150. https://doi.org/10.1111/j.1600-0854.2010.01088.x. | spa |
dc.relation.references | Ho CY, Choy CH, Wattson CA, Johnson DE, Botelho RJ. 2015. The Fab1/PIKfyve phosphoinositide phosphate kinase is not necessary to maintain the pH of lysosomes and of the yeast vacuole. J Biol Chem 290:9919 –9928. https://doi.org/10.1074/jbc.M114.613984. | spa |
dc.rights.creativecommons | Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) | spa |
dc.subject.proposal | Candida albicans | spa |
dc.subject.proposal | Chemical screening | spa |
dc.subject.proposal | PH dynamics | spa |
dc.subject.proposal | Vacuoles | spa |
dc.contributor.corporatename | American Society for Microbiology. | spa |
dc.type.content | Text | spa |
dc.type.redcol | http://purl.org/coar/resource_type/c_2df8fbb1 | spa |
oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |