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dc.contributor.authorTournu, Helénespa
dc.contributor.authorLuna Tapia, Arturospa
dc.contributor.authorPeters, Brian M.spa
dc.contributor.authorPalmer, Glen E.spa
dc.coverage.spatialColombiaspa
dc.date.accessioned2019-09-24T21:38:38Zspa
dc.date.available2019-09-24T21:38:38Zspa
dc.date.issued2019-09-24spa
dc.identifier.citationTournu, H., Luna, A., Peters, B.M. & Palmer, G.E. (2017). In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. Revista mSphere . 2(4).spa
dc.identifier.issn1098-6596spa
dc.identifier.urihttps://repositorio.unisucre.edu.co/handle/001/791spa
dc.descriptionArtículo digital.spa
dc.description.abstractEnvironmental or chemically induced stresses often trigger physiological responses that regulate intracellular pH. As such, the capacity to detect pH changes in real time and within live cells is of fundamental importance to essentially all aspects of biology. In this respect, pHluorin, a pH-sensitive variant of green fluorescent protein, has provided an invaluable tool to detect such responses. Here, we report the adaptation of pHluorin2 (PHL2), a substantially brighter variant of pHluorin, for use with the human fungal pathogen Candida albicans. As well as a cytoplasmic PHL2 indicator, we describe a version that specifically localizes within the fungal vacuole, an acidified subcellular compartment with important functions in nutrient storage and pH homeostasis. In addition, by means of a glycophosphatidylinositolanchored PHL2-fusion protein, we generated a cell surface pH sensor.eng
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherEstados Unidos: mSphere, 2017.spa
dc.relation.ispartofArticulo de revistaspa
dc.rightsDerechos Reservados - Universidad de Sucre, 2019spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourcehttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497024/spa
dc.titleIn Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.spa
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.identifier.doi10.1128/mSphere.00276-17spa
dc.relation.referencesMiesenböck G, De Angelis DA, Rothman JE. 1998. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394:192–195. https://doi.org/10.1038/28190.spa
dc.relation.referencesBencina M. 2013. Illumination of the spatial order of intracellular pH by genetically encoded pH-sensitive sensors. Sensors 13:16736 –16758. https://doi.org/10.3390/s131216736.spa
dc.relation.referencesLiu NN, Köhler JR. 2016. Antagonism of fluconazole and a proton pump inhibitor against Candida albicans. Antimicrob Agents Chemother 60: 1145–1147. https://doi.org/10.1128/AAC.02043-15.spa
dc.relation.referencesProsser DC, Whitworth K, Wendland B. 2010. Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras. Traffic 11:1141–1150. https://doi.org/10.1111/j.1600-0854.2010.01088.x.spa
dc.relation.referencesHo CY, Choy CH, Wattson CA, Johnson DE, Botelho RJ. 2015. The Fab1/PIKfyve phosphoinositide phosphate kinase is not necessary to maintain the pH of lysosomes and of the yeast vacuole. J Biol Chem 290:9919 –9928. https://doi.org/10.1074/jbc.M114.613984.spa
dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.subject.proposalCandida albicansspa
dc.subject.proposalChemical screeningspa
dc.subject.proposalPH dynamicsspa
dc.subject.proposalVacuolesspa
dc.contributor.corporatenameAmerican Society for Microbiology.spa
dc.type.contentTextspa
dc.type.redcolhttp://purl.org/coar/resource_type/c_2df8fbb1spa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa


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