Comparación y utilidad de tres métodos de extracción de ADN a partir de potenciales vectores de enfermedades rickettsiales (Acari: Ixodidae) en Colombia.
Guevara Vega, Marco | 2019-11-08
The study of diseases transmitted for tick as rickettsia is of great interest to medical and veterinary level and requires the use of extracts of good quality DNA for subsequent analysis of amplified genes of these pathogens. In this study, three methods were compared DNA extraction from ticks complex Rhipicephalus sanguineus based on: chaotropic guanidine thiocyanate (method 1), high salt (method 2) and an extraction method based on commercially available columns (Method 3). The DNA was quantified and the quality of each extract in the amplification of the 16S gene ticks (360pb) was evaluated, a nonparametric Kruskal-Wallis one-way analysis was performed, while comparing the amplification efficiency of mitochondrial gene was performed by Dunn test range. Method 3 allowed the amplification of the 16S gene of ticks in 100% (30/30) followed by method 1 with 93.33% (28/30) and finally with method 2 86.66% (26/30). The three methods are efficient at extracting genetic material of high quality tick-borne studies since their performance in amplification of mitochondrial genes tick agents.