Dengue del virus 1 aislado de la poliproteína Ov07, región de la proteína E de la envoltura, gen (POLY), CD parcial

Camacho Burgos, Erwin Yesid | 2019-04-10

Actividad Leishmanicida de annona purpurea

Cárdenas, Dary Luz | 2019-09-16

The ethanolic fraction of total extract of Annona purpurea was evaluated in vitro against amastigotes of Leishmania panamensis and the cell line U937. This fraction was more active as leishmanicidal than Glucantime®.

A new species of sycorax(Diptera, psychodidae, sycoracinae) from the pacific coast of Colombia

Bejarano, Eduar Elías | 2019-09-16

A new species of Sycorax (Diptera, Psychodidae,sycoracinae) from the pacific coast of Colombia.Sycorax utriensis n. sp. is described and illustrated from a male specimen collected with a CDC light trap in the parque Nacional Natural Ensenada de Utría,on the pacific Coast of Colombia.Five species of sycorax are now known from Colombia,S.andicola Young,1979,S.colombiensis Young,1979,S.trispinosa Young,1979 and S. utriensis n.sp.

Comparación de tres métodos de extracción de ADN a partir de garrapatas duras (Acari: Ixodidae)

Guevara Vega, Marco | 2019-09-18

The aim of the present investigation was to compare the performance of three DNA extraction methods (salts, columns and guanidine thiocyanate) from ticks for studies of pathogen detection and molecular systematics of ticks. DNA extraction performance was measured by multiple comparisons on 30 tick samples and assessment of quality of the DNA extract through PCR amplification of 16S mitochondrial ticks gene. The presence of Rickettsia, Ehrlichia, Anaplasma and Babesia.

Brote fiebre por virus Chikungunya en Ovejas, Sucre, septiembre de 2014.

Castillo Pabón, José Orlando | 2019-09-18

La fiebre Chikungunya es una enfermedad endémica en África, Asia e India y más recientemente en América. Se manifiesta por fiebre, poliartralgias y artritis. Se describe a continuación un brote de esta enfermedad detectado en el mes de septiembre de 2014 en el municipio de Ovejas ( Sucre ), haciendo énfasis en las manifestaciones clínicas, el tratamiento médico implementado y algunos antecedentes epidemiológicos. Las artralgias con 96,0% y la fiebre con 92,0% fueron los signos y síntomas más comunes.

Detección de infección natural por trypanosoma cruzi (trypanosomatidae) en triatominos del municipio de Colosó, Colombia

Ayala Hoyos, Carolina Julieth | 2019-09-19

The report of triatomines infected with Trypanosoma cruzi in a wild area of the municipality of Colosó, made it necessary to determine the species of vectors near the houses in the village Jorro, because it is the closest rural settlement to this finding. The presence of triatomine species with epidemiological importance was reported by first time, with a high percentage of infection with the parasite, in addition to expanding the distribution of these vectors in the department of Sucre. The researchers captured insects in 13 dwellings through active search, community surveillance and light traps.

Development of Envelope Protein Antigens To Serologically Differentiate Zika Virus Infection from Dengue Virus Infection

Lakshmanane, Premkumar | 2019-09-19

Zika virus (ZIKV) is an emerging flavivirus that can cause birth defects and neurologic complications. Molecular tests are effective for diagnosing acute ZIKV infection, although the majority of infections produce no symptoms at all or present after the narrow window in which molecular diagnostics are dependable. Serology is a reliable method for detecting infections after the viremic period; however, most serological assays have limited specificity due to cross-reactive antibodies elicited by flavivirus infections. Since ZIKV and dengue virus (DENV) widely cocirculate, distinguishing ZIKV infection from DENV infection is particularly important for diagnosing individual cases or for surveillance to coordinate public health responses.

Babesia bigemina en bovinos del municipio Los Palmitos (Sucre, Colombia)

Martínez Mercado, María Rebeca | 2019-09-19

Bovine babesiosis is a disease that usually causes losses in the livestock sector, so it is necessary to diagnose early the haemoparasite in cattle. The aim of this study was the detection, by duplex PCR, of Babesia species that are present in cattle of the municipality of Los Palmitos in the department of Sucre, Colombia.

Variabilidad genética de Aedes aegypti en el departamento de Sucre, Colombia, mediante el análisis de la secuencia de nucleótidos del gen mitocondrial ND4.

Atencia, María Claudia | 2019-09-20

Aedes aegypti is the most important mosquito species in America for the transmission of viruses of dengue, Zika, Chikungunya and yellow fever. Ecological factors as well as chemical controls can affect the genetic composition of Ae. aegypti populations, which is why its genetic characterization is necessary.

Mycobacterium avium subespecie paratuberculosis en heces de bovinos del municipio de Sincelejo, Sucre, Colombia

Caraballo Blanco, Libardo | 2019-09-20

The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle faeces from the municipality of Sincelejo, Sucre, Colombia. A direct bacteriological study was performed by microscopy techniques and polymerase chain reaction (PCR) for pools of faeces with two molecular markers, one specific for MAP (IS900) and another based on 16S of the genus Mycobacterium. The presence of acid-fast bacilli occurred in 21.1% of the faecal samples stained with Ziehl Neelsen. Three pools were positive with IS900 and three with 16S. The sequencing indicated the presence of three species of bacteria: MAP, Mycobacterium abscessus and Dietzia timorensis. The presence of MAP in the department of Sucre is identified for the first time.

Primer registro del flebotomíneo Warileya (Hertigia) hertigi en Antioquia, Colombia.

Bejarano, Eduar Elías | 2019-09-20

The genus Warileya is one of the least-known taxa of sandflies, comprising only nine species, i.e., W. (Warileya) phlebotomanica, W. (Hertigia) hertigi, W. (W.) rotundipennis, W. (W.) nigrosacculus, W. (W.) yungasi, W. (W.) fourgassiensis, W. (W.) lumbrerasi, W. (W.) euniceae and W. (W.) leponti.

Aislamiento de Leishmania infantum a partir de Canis familiaris en área urbana del Caribe colombiano

Rivero Rodríguez, Matilde Elena | 2019-09-20

The isolation of parasites of the genus Leishmania is reported from a popliteal ganglion biopsy obtained from a dog with canine visceral leishmaniasis. The dog lived in the urban area of the municipality of Ovejas, Sucre, Colombia. The Indirect Immunofluorescence test was positive for anti-Leishmania spp antibodies up to a dilution of 1/128. The typing of L. infantum was performed by isoenzyme electrophoresis and by 924 Rev Inv Vet Perú 2018; 29(3): 923-930 M. Rivero et al. analysis of the sequence of the cytochrome b gene and the nuclear gene that codes for the heat shock protein of 70 Kda (Hsp70). The present finding is the first confirmation of infection by L. infantum in canines in the urban area of the Colombian Caribbean.

Modelos potenciales de distribución geográfica y climática del complejo Amblyomma cajennense (Acari: Ixodidae), potencial vector de Rickettsia rickettsii en Colombia.

Acevedo Gutiérrez, Leidy Y. | 2019-09-23

The species of the Amblyoma cajennense complex, potential vectors involved in the transmission of Rickettsia rickettsii in Central and South America, have a wide geographical distribution in the Neotropics. In Colombia, the knowledge about these species, their distribution, and many aspects of their ecology is still limited.

Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks.

Pérez Pérez, Juan C. | 2019-09-24

Background: Ehrlichia and Rickettsia are two major rickettsial genera transmitted by ticks that affect a number of wild and domestic animal species and human populations around the world.

Evaluación de las prácticas sanitarias que ejecutan algunos hatos ganaderos de la subregión Montes de María (Sucre, Colombia)

Contreras Gómez, María José | 2019-09-24

Animal health is one of the aspects most important fact in production processes, more in the livestock sector where losses generated by the presence of disease in herds is both economic and health problem because everything related is risk. In this paper, it was intended to know by personal communication of the owners of cattle, the main diseases affecting cattle in the sub-region Montes de Maria. An evaluative survey was applied to herd owners in the sub-region and the data reported by different governmental entities were reviewed.

Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.

Rybak, Jeffrey M. | 2019-09-24

Among emerging non-albicans Candida species, Candida parapsilosis is of particular concern as a cause of nosocomial bloodstream infections in neonatal and intensive care unit patients. While fluconazole and echinocandins are considered effective treatments for such infections, recent reports of fluconazole and echinocandin resistance in C. parapsilosis indicate a growing problem. The present study describes a novel mechanism of antifungal resistance in this organism affecting susceptibility to azole and echinocandin antifungals in a clinical isolate obtained from a patient with prosthetic valve endocarditis. Transcriptome analysis indicated differential expression of several genes in the resistant isolate, including upregulation of ergosterol biosynthesis pathway genes ERG2, ERG5, ERG6, ERG11, ERG24, ERG25, and UPC2.

Presence of Leptospire spp. in urban bats from Sincelejo, Sucre, Colombia.

Victoria, Robin Jesús | 2019-09-24

Leptospirosis is one of the most frequent zoonoses worldwide and occurs in tropical, subtropical and temperate areas. The genus Leptospira comprises saprophytic and pathogenic species. The latter isolated from several animals that serve as reservoirs and carriers. The presence of bats in urban areas has increased for various reasons, profusion of plants, lack of predators, presence of luminaries, which has increased the chances of contact between bats, humans and/or pets. The aim of this work was to investigate the presence of pathogenic Leptospira in bats captured in the city of Sincelejo, using the molecular PCR technique. Mist nets were used to capture the bats and these were sacrificed using ether to obtain samples of renal tissue. A fragment of the LipL32 gene was amplified by PCR technique. We identified three families of bats amongst our sample and 26% of them presented pathogenic Leptospira DNA. This represents a great risk to the community in this region.

In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.

Tournu, Heléne | 2019-09-24

Environmental or chemically induced stresses often trigger physiological responses that regulate intracellular pH. As such, the capacity to detect pH changes in real time and within live cells is of fundamental importance to essentially all aspects of biology. In this respect, pHluorin, a pH-sensitive variant of green fluorescent protein, has provided an invaluable tool to detect such responses. Here, we report the adaptation of pHluorin2 (PHL2), a substantially brighter variant of pHluorin, for use with the human fungal pathogen Candida albicans. As well as a cytoplasmic PHL2 indicator, we describe a version that specifically localizes within the fungal vacuole, an acidified subcellular compartment with important functions in nutrient storage and pH homeostasis. In addition, by means of a glycophosphatidylinositolanchored PHL2-fusion protein, we generated a cell surface pH sensor.

An azole tolerant endosomal trafficking mutant of Candida albicans is susceptible to azole treatment in a mouse model of vaginal candidiasis.

Peters, Brian M. | 2019-09-24

We recently reported that a Candida albicans endosomal trafficking mutant continues to grow after treatment with the azole antifungals. Herein, we report that the vps21Δ/Δ mutant does not have a survival advantage over wild-type isolates after fluconazole treatment in a mouse model of vaginal candidiasis. Furthermore, loss of VPS21 does not synergize with established mechanisms of azole resistance, such as overexpression of efflux pumps or of Erg11p, the target enzyme of the azoles. In summary, although loss of VPS21 function enhances C. albicans survival after azole treatment in vitro, it does not seem to affect azole susceptibility in vivo.

DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites.

Vivero, Rafael José | 2019-09-25

Although phlebotomine sand flies breeding sites have been identified and recorded by several studies, the microhabitats exploited by these insects remain little-known and hard to find. In this context, the difficulty of finding immature stages, and the limited number of taxonomic studies to identify immature stages of phlebotomine sand flies, are considered the major obstacles when attempting a complete inventory of Lutzomyia species. The objective of this study is to validate Cytochrome Oxidase I (Barcode region) as a marker for the identification of immature stages of Lutzomyia species recovered from natural breeding sites in Colombia. Among 142 collected sand flies, 18 immature individuals that did not complete their life cycle were identified to species level through sequencing of the COI gene. Values of K2P genetic distance between 0.002–0.031 allowed the identification of larvae at species level. The bootstrap support values (96%) in the Neighbor-Joining dendrogram were consistent for the majority of the established MOTUS of Lutzomyia atroclavata, Lutzomyia micropyga, Lutzomyia serrana, Lutzomyia cayennensis, Lutzomyia rangeliana, Lutzomyia shannoni and some species of the genus Brumptomyia. The COI gene is validated as a marker for the identification of immature stages of the genus Lutzomyia.