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Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.

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dc.contributor.authorPaternina, Luís E.spa
dc.contributor.authorVerbel Vergara, Danielspa
dc.contributor.authorRomero Ricardo, Luísspa
dc.contributor.authorPérez Doria, Alveirospa
dc.contributor.authorPaternina Gómez, Margaretspa
dc.contributor.authorMartínez, Lilyspa
dc.contributor.authorBejarano, Eduar E.spa
dc.coverage.spatialColombiaspa
dc.date.accessioned2019-11-06T16:08:15Zspa
dc.date.available2019-11-06T16:08:15Zspa
dc.date.issued2019-11-06spa
dc.descriptionArtículo digital.spa
dc.description.abstractIdentification of the bloodmeal sources of phlebotomine sand flies is fundamental to determining which species are anthropophilic and understanding the transmission of Leishmania parasites in natural epidemiological settings. The objective of this study was to identify sand fly bloodmeals in the mixed leishmaniasis focus of the department of Sucre, northern Colombia. In all 141 engorged female sand flies were analyzed, after being captured in intradomiciliary, peridomiciliary and extradomiciliary habitats with Shannon and CDC traps and by active searching in diurnal resting sites. Bloodmeals were identified by sequencing and analysis of a 358 bp fragment of the mitochondrial gene Cytochrome b (CYB) and a 330 bp fragment of the nuclear gene prepronociceptin (PNOC). Using both genes 105 vertebrate bloodmeals were identified, with an efficiency of 72% for CYB but only 7% for PNOC. Ten species of vertebrates were identified as providing bloodmeal sources for eight sand fly species: Homo sapiens (Lutzomyia evansi, Lu. panamensis, Lu. micropyga, Lu. shannoni and Lu. atroclavata), Equus caballus (Lu. evansi, Lu. panamensis and Lu. cayennensis cayennensis), Eq. asinus (Lu. evansi and Lu. panamensis), Bos taurus 4 (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Tamandua mexicana (Lu. shannoni and Lu. trinidadensis), Proechimys guyanensis (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Mabuya sp. (Lu. micropyga), Sus scrofa (Lu. evansi and Lu. gomezi) and Gallus gallus (Lu. evansi). Cattle, donkeys, humans and pigs were significantly more important than other animals (P = 0.0001) as hosts of Lu. evansi, this being the most abundant sand fly species. The five Lutzomyia species in which blood samples of human origin were detected included Lu. micropyga and Lu. atroclavata, constituting the first evidence of anthropophily in both species.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.citationPaternina, L.E., Verbel, D., Romero, L., Pérez, A., Paternina, M., Martínez, L. & Bejarano, E.E. (2015). Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Acta Tropica. 153: 86 - 92.spa
dc.identifier.doihttp://dx.doi.org/doi:10.1016/j.actatropica.2015.10.005.spa
dc.identifier.issn0001-706X.spa
dc.identifier.urihttps://repositorio.unisucre.edu.co/handle/001/956spa
dc.language.isoengspa
dc.publisherReino Unido: Acta Tropica, 2015.spa
dc.relation.ispartofRevistaspa
dc.relation.referencesAlexander, B., 2000. Sampling methods for phlebotomine sand flies (Diptera: Psychodidae). Med. Vet. Entomol. 14, 1–13.spa
dc.relation.referencesAlexander, B., de Carvalho, R.L., McCallum, H., Pereira, M.H., 2002. Role of the domestic chicken (Gallus gallus) in the epidemiology of urban visceral leishmaniasis in Brazil. Emerg. Infect. Dis. 8, 1480–1485.spa
dc.relation.referencesAlexander, B., Lozano, C., Barker, D.C., McCann, S.H., Adler, G.H., 1998. Detection of Leishmania (Viannia) braziliensis complex in wild mammals from Colombian coffee plantations by PCR and DNA hybridization. Acta Trop. 69, 41–50.spa
dc.relation.referencesAlvar, J., Vélez, I.D., Bern, C., Herrero, M., Desjeux, P., Cano, J., Jannin, J., den Boer, M., 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7, e35671. doi:10.1371/journal.pone.0035671.spa
dc.relation.referencesBejarano E.E., Estrada L.G., 2015. Family Psychodidae, in: Wolff, M., Nihei, S.S., Pérez, S.P., & de Carvalho, C.J.B. (Eds.), Catalogue of Diptera of Colombia. Zootaxa, In Press.spa
dc.rightsDerechos Reservados - Universidad de Sucre, 2019spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourcehttps://www.ncbi.nlm.nih.gov/pubmed/26464046.spa
dc.subject.proposalLutzomyiaspa
dc.subject.proposalSandfliesspa
dc.subject.proposalBloodmealsspa
dc.subject.proposalMolecular analysisspa
dc.subject.proposalLeishmaniasisspa
dc.subject.proposalColombiaspa
dc.titleEvidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.spa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa

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