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DNA barcoding to identify species of phlebotomine sand fly (Diptera: Psychodidae) in the mixed leishmaniasis focus of the Colombian Caribbean.

dc.contributor.authorRomero Ricardo, Luisspa
dc.contributor.authorLastre Meza, Nataliaspa
dc.contributor.authorBejarano, Eduar E.spa
dc.contributor.authorPérez Doria, Alveirospa
dc.coverage.spatialColombiaspa
dc.date.accessioned2019-10-01T14:23:02Zspa
dc.date.available2019-10-01T14:23:02Zspa
dc.date.issued2019-09-27spa
dc.descriptionArtículo digital.spa
dc.description.abstractIdentification of the species of phlebotomine sand flies present in each focus of leishmaniasis is necessary to incriminate vectors and implement vector control strategies. Although the cytochrome oxidase I (COI) gene has been proposed as a barcode for the identification of animal species, less than 20% of New World phlebotomines have been characterized to date. In this study DNA barcoding was used to identify phlebotomine species of the mixed leishmaniasis focus in the Colombian Caribbean by means of three evolutionary models: Kimura’s two parameter (K2P) nucleotide substitution model, that of (Tamura and Nei, 1993) (TN93) and proportional sequence divergence (p-distances). A 681 bp sequence of the COI gene was obtained from 66 individuals belonging to 19 species of the genus Lutzomyia (Lu. abonnenci, Lu. atroclavata, Lu. bicolor, Lu. carpenteri, Lu. cayennensis cayennensis, Lu. dubitans, Lu. evansi, Lu. gomezi, Lu. gorbitzi, Lu. longipalpis, Lu. micropyga, Lu. migonei, Lu. panamensis, Lu. (Psathyromyia) sp., Lu. rangeliana, Lu. serrana, Lu. shannoni, Lu. trinidadensis and Lu. venezuelensis) and one of Brumptomyia (B. mesai).eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.citationRomero, L., Lastre, N., Pérez, A. & Bejarano, E.E. (2016). DNA barcoding to identify species of phlebotomine sand fly (Diptera: Psychodidae) in the mixed leishmaniasis focus of the Colombian Caribbean. Acta tropica. 159: 125-131.spa
dc.identifier.doi10.1016/j.actatropicaspa
dc.identifier.issn0001-706Xspa
dc.identifier.urihttps://repositorio.unisucre.edu.co/handle/001/807spa
dc.language.isoengspa
dc.publisherPaíses Bajos: Revista Acta Tropica, 2016.spa
dc.relation.ispartofRevistaspa
dc.relation.referencesAbonnenc, E., Léger, N., 1976. Sur une classification rationnelle des diptères Phlebotomidae. Cah. ORSTOM, ser. Ent. Med. Parasitol. 14, 69–78.spa
dc.relation.referencesAltschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., 1990. Basic local alignment search tool. J. Mol. Biol. 215, 403–410. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., 1990. Basic local alignment search tool. J. Mol. Biol. 215, 403–410.spa
dc.relation.referencesArtemiev, M.M., 1991. A classification of the subfamily Phlebotominae. Parassitologia 33 (Suppl), 69–7.spa
dc.relation.referencesAzpurua, J., De La Cruz, D., Valderama, A., Windsor, D., 2010. Lutzomyia sand fly diversity and rates of infection by Wolbachia and an exotic Leishmania species on Barro Colorado Island, Panama. PLoS Negl. Trop. Dis. 4, e627.spa
dc.relation.referencesBarretto, M.P., 1955. Sobre a sistemática da subfamilia Phlebotominae Rondani (Diptera, Psychodidae). Rev. Bras. Entomol. 3, 173–190.spa
dc.rightsDerechos Reservados - Universidad de Sucre, 2019spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourcehttps://www.sciencedirect.com/science/articlespa
dc.subject.proposalLutzomyiaspa
dc.subject.proposalSand fliesspa
dc.subject.proposalDNA barcodingspa
dc.subject.proposalCytochrome oxidase I (COIspa
dc.subject.proposalSpecies identificationspa
dc.titleDNA barcoding to identify species of phlebotomine sand fly (Diptera: Psychodidae) in the mixed leishmaniasis focus of the Colombian Caribbean.spa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa

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