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Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia.

Camacho, Erwin; Paternina Gómez, Margaret; Blanco Tuirán, Pedro J.; Osorio, Jorge E.; Aliota, Matthew T. (Estados Unidos: Emerging Infectious Diseases , 2016., 2019-09-27)

During October–November 2015, a total of 22 patients received a presumptive diagnosis of an acute viral illness by emergency department physicians at the Centro de Diagnostico Medico-Universidad de Sucre in Sincelejo. The ...
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series.

Gómez Carmago, Doris E.; Blanco, Pedro; Mora García, Gustavo; Paternina, Margareth; Caraballo, Libardo (Estados Unidos, American Journal of Tropical Medicine and Hygiene , 2015., 2019-10-30)

Leptospirosis is a public health problem on the Colombian Caribbean Coast. In Cartagena, a major city in this region, this disease is endemic with high annual lethality. In clinical practice, differential diagnosis of ...
Presence of Leptospire spp. in urban bats from Sincelejo, Sucre, Colombia.

Victoria, Robin Jesús; Iriarte, Lilia Judith; Sampedro, Alcides (Coden, USA: International Journal of PharmTech Research, 2018., 2019-09-24)

Leptospirosis is one of the most frequent zoonoses worldwide and occurs in tropical, subtropical and temperate areas. The genus Leptospira comprises saprophytic and pathogenic species. The latter isolated from several ...
Zika virus like particles elicit protective antibodies in mice.

Salvo, Mauricio A; Kingstad Bakke, Brock; Salas Quinchucua, Cristhian; Camacho, Erwin; Osorio, Jorge E. (California, Estados Unidos: PLoS Neglected Tropical Diseases , 2018., 2019-10-29)

Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated ...

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Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia.
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Camacho, Erwin | 2019-09-27

During October–November 2015, a total of 22 patients received a presumptive diagnosis of an acute viral illness by emergency department physicians at the Centro de Diagnostico Medico-Universidad de Sucre in Sincelejo. The patients began treatment for a dengue-like illness, and blood samples were obtained for diagnosis. The samples were analyzed at the Universidad de Sucre by using reverse transcription PCR (RT-PCR) to detect DENV, CHIKV, or Zika virus. Viral RNA was extracted from the serum samples by using the ZR Viral RNA Kit (Zymo Research, Irvine, CA, USA); reverse transcription was performed by using the Protoscript First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). Amplicons were produced by using the OneTaq Quick-Load 2X Master Mix (New England Biolabs) with primers specific to DENV (7), CHIKV (forward: 5′-CGCCAACATTCTGCTTACAC-3′; reverse: 5′-AGGATGCCGGTCATTTGAT-3′), and Zika virus. T

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Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series.
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Gómez Carmago, Doris E. | 2019-10-30

Leptospirosis is a public health problem on the Colombian Caribbean Coast. In Cartagena, a major city in this region, this disease is endemic with high annual lethality. In clinical practice, differential diagnosis of leptospirosis with others icteric febrile syndromes is crucial to an appropriated therapeutic approach. Nevertheless, high complexity hospitals employ only serological test to confirm suspicious cases, with an elevated frequency of undetermined results. Therefore, the aim of this study was to apply molecular techniques in patients under leptospirosis suspicion. It was carried out a case series study with adults in 2013 last trimester. Patients with clinical manifestations and positive/undetermined results in serological test for leptospirosis were included. We used Polymerase Chain Reaction to detect Leptospira spp from blood samples with specific primers that amplify a DNA segment from the lipl32 gen. A total of 9 patients were included (3 women, 6 men). IgM test for leptospirosis was positive in 5 subjects and undetermined in 4 others. A 423pb product was expected, however only 3 samples showed an amplicon of 358pb. When those DNA fragments were sequenced, none of them were part of Leptospira spp. genome. Thereafter, samples were amplified for Riskettsia spp. by nested PCR for the OmpB gene. Seven samples were positive (420pb amplicon). These samples were also positive for Rickettsia spp 17KDa gene (230pb amplicon).

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Presence of Leptospire spp. in urban bats from Sincelejo, Sucre, Colombia.
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Victoria, Robin Jesús | 2019-09-24

Leptospirosis is one of the most frequent zoonoses worldwide and occurs in tropical, subtropical and temperate areas. The genus Leptospira comprises saprophytic and pathogenic species. The latter isolated from several animals that serve as reservoirs and carriers. The presence of bats in urban areas has increased for various reasons, profusion of plants, lack of predators, presence of luminaries, which has increased the chances of contact between bats, humans and/or pets. The aim of this work was to investigate the presence of pathogenic Leptospira in bats captured in the city of Sincelejo, using the molecular PCR technique. Mist nets were used to capture the bats and these were sacrificed using ether to obtain samples of renal tissue. A fragment of the LipL32 gene was amplified by PCR technique. We identified three families of bats amongst our sample and 26% of them presented pathogenic Leptospira DNA. This represents a great risk to the community in this region.

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Zika virus like particles elicit protective antibodies in mice.
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Salvo, Mauricio A | 2019-10-29

Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated with a wide range of congenital abnormalities. To date, no vaccines or antivirals are publicly available. We developed Zika virus-like particles (VLPs) and evaluated their immunogenicity and protective efficacy in mouse models. ZIKV VLPs (ZIKVLPs) formulated with alum were injected into 6-8-week-old interferon deficient AG129 mice as well as wild type BALB/c mice. Control mice received PBS/alum. Animals were challenged with 200 PFU (>1000 AG129 LD50s) of ZIKV strain H/PF/2013. All vaccinated mice survived with no morbidity or weight loss while control animals either died at 9 days post challenge (AG129) or had increased viremia (BALB/c). Neutralizing antibodies were observed in all ZIKVLP vaccinated mice. The role of neutralizing antibodies in protecting mice was demonstrated by passive transfer. Our findings demonstrate the protective efficacy of the ZIKVLP vaccine and highlight the important role that neutralizing antibodies play in protection against ZIKV infection.

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