Comparación de tres métodos de extracción de ADN a partir de garrapatas duras (Acari: Ixodidae)
Guevara Vega, Marco | 2019-09-18
The aim of the present investigation was to compare the performance of three DNA extraction methods (salts, columns and guanidine thiocyanate) from ticks for studies of pathogen detection and molecular systematics of ticks. DNA extraction performance was measured by multiple comparisons on 30 tick samples and assessment of quality of the DNA extract through PCR amplification of 16S mitochondrial ticks gene. The presence of Rickettsia, Ehrlichia, Anaplasma and Babesia.