Examinando por Materia "Sand flies"
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Publicación Acceso abierto DNA barcoding to identify species of phlebotomine sand fly (Diptera: Psychodidae) in the mixed leishmaniasis focus of the Colombian Caribbean.(Países Bajos: Revista Acta Tropica, 2016., 2019-09-27) Romero Ricardo, Luis; Lastre Meza, Natalia; Bejarano, Eduar E.; Pérez Doria, AlveiroIdentification of the species of phlebotomine sand flies present in each focus of leishmaniasis is necessary to incriminate vectors and implement vector control strategies. Although the cytochrome oxidase I (COI) gene has been proposed as a barcode for the identification of animal species, less than 20% of New World phlebotomines have been characterized to date. In this study DNA barcoding was used to identify phlebotomine species of the mixed leishmaniasis focus in the Colombian Caribbean by means of three evolutionary models: Kimura’s two parameter (K2P) nucleotide substitution model, that of (Tamura and Nei, 1993) (TN93) and proportional sequence divergence (p-distances). A 681 bp sequence of the COI gene was obtained from 66 individuals belonging to 19 species of the genus Lutzomyia (Lu. abonnenci, Lu. atroclavata, Lu. bicolor, Lu. carpenteri, Lu. cayennensis cayennensis, Lu. dubitans, Lu. evansi, Lu. gomezi, Lu. gorbitzi, Lu. longipalpis, Lu. micropyga, Lu. migonei, Lu. panamensis, Lu. (Psathyromyia) sp., Lu. rangeliana, Lu. serrana, Lu. shannoni, Lu. trinidadensis and Lu. venezuelensis) and one of Brumptomyia (B. mesai).Publicación Acceso abierto Genetic analysis of a recently detected urban population of Lutzomyia evansi (Diptera: Psychodidae) in Colombia.(Buenos Aires, Argentina: Revista de la Sociedad Entomológica Argentina, 2009., 2019-10-16) Bejarano, Eduar Elías; Rojas, Winston; Uribe, Sandra; Vélez, Iván Darío; Porter, Cherles H.; Sociedad Entomológica Argentina.Lutzomyia evansi (Núñez-Tovar) is the vector of the parasite Leishmania infantum in rural zones of Northern Colombia. An attempt was made to determine the origin of a recently detected urban population of Lutzomyia evansi by genetically characterizing specimens from seven geographically distinct localities in the Colombian Caribbean. Insect specimens were collected in rural and urban environments of areas endemic for visceral leishmaniasis or free of the disease. Nine polymorphic sites, nine nucleotide haplotypes and a single aminoacid haplotype were found within the 315 bp fragment sequenced, corresponding to the 3’ end of the cytochrome b mitochondrial gene. Paired genetic distances between the haplotypes, estimated with the Kimura two-parameters model, varied from 0,0032-0,0194. Analysis revealed low genetic variability between specimens from urban and rural localities. Several of the sand flies collected in the city of Sincelejo (department of Sucre), where autochthonous visceral leishmaniasis cases have appeared in recent years, were genetically similar to those of a rural focus of the disease (El Contento, on the neighboring department of Córdoba). The epidemiological implications of this finding for Leishmania infantum transmission in the Colombian Caribbean are discussed.