Examinando por Materia "Garrapatas"
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Publicación Acceso abierto Canis familiaris, un nuevo hospedero de Ornithodoros (A.) puertoricensis FOX, 1947 (Acari: Ixodida) en Colombia.(Bogotá, Colombia: Acta Biológica Colombiana , 2009., 2019-10-15) Paternina, Luis E.; Díaz Olmos, Yirys; Paternina Gómez, Margaret; Eduar E., BejaranoTicks are very important from the biomedical point of view, by their hematophagic activity and their role in the transmission of pathogenic microorganisms to man and animals. The main goal of this work was to establish the tick species parasiting dogs in three rural localities of the Colombian Caribbean. From August to December 2006, an active search of ticks on dogs was carried out in the localities of El Campín, Sabanas del Potrero and Escobar Arriba, department of Sucre. The collected ticks were preserved into eppendorf tubes with 70% ethanol, and identified using standard morphological keys for each family. Argasid species were determined by measuring external morphological characters. Of 134 examined dogs in the three localities, 50 were found infested by ticks, representing a infestation rate of 37,3%. A total of 420 ticks were collected from dogs and identified as Rhipicephalus sanguineus, Rhipicephalus (Boophilus) microplus, and Amblyomma ovale of the Ixodidae family, and Ornithodoros puertoricensis of the Argasidae family. R. sanguineus was the predominant species (92,1%) in the stages of larva, nymph and adult, following by O. puertoricensis larvae recorded in low numbers in the three regions sampled. The tick O. puertoricensis is recorded for the first time as ectoparasite of domestic dogs in America. Additionally, the presence of this tick species is confirmed in Colombia.Publicación Acceso abierto Comparación y utilidad de las regiones mitocondriales de los genes 16S y COX1 para los análisis genéticos en garrapatas (Acari: Ixodidae).(Bogotá, Colombia : Revista biomédica, 2016., 2019-10-01) Paternina, Luis Enrique; Verbel Vergara, Daniel; Bejarano, Eduar ElíasIn recent decades the analysis of mitochondrial genes has been used for population and phylogenetic studies of ticks allowing many advances in their systematics. Mitochondrial ribosomal 16S (16S) subunit is one of the most frequently used among those genes available for tick analysis, whereas cytochrome oxidase gene 1 (COX1) has recently been used and proposed as an alternative to the traditional 16S gene marker. The main objective of the present investigation is To evaluate the usefulness of 16S and COX1 in genetic studies of ticks by analyzing sequences of three species commonly found in the Caribbean region of Colombia.Publicación Acceso abierto Detección e identificación molecular de Babesia (Piroplasma: Babesiidae) en garrapatas asociadas a animales domésticos del departamento de Sucre, Colombia(Universidad de Sucre, 2019) Guevara Vega, Marco Fidel; Paternina Tuirán, LuisLa babesiosis es causada por parásitos protozoarios intraeritrociticos que son transmitidos por especies de garrapatas, capaces de infectar animales domésticos y salvajes. Estos protozoos del género Babesia han causado gran pérdida económica dentro de la industria ganadera debido a la mortalidad, morbilidad y los altos costos que implica la atención veterinaria. En Colombia, diversos estudios han permitido determinar que la babesiosis especialmente la bovina tiene una gran repercusión económica y sanitaria, sin embargo, trabajos dirigidos sobre la ixodofauna relacionada como el vector de la enfermedad poco se ha realizado. Por esta razón, en el presente trabajo de investigación, se identificó protozoarios del genero Babesia asociados a garrapatas en ambientes rurales del departamento de Sucre. 1.084 garrapatas (487 R. sanguineus, 336 D. nitens, 251 R. microplus, 7 A. mixtum y 3 A. auricularium) fueron colectadas de diferentes animales domésticos organizados en 350 grupos de ectoparásitos, donde 165 grupos eran de R. sanguineus, 97 D. nitens, 83 R. microplus, 4 A. mixtum y 1 A. auricularium. A estos grupos de garrapatas se les realizó extracción de ADN, luego una PCR anidada de la región ITS1 y PCR del gen 18S ARNr de especies de Babesia. Posteriormente la secuenciación y análisis genético que permitió la identificación de los parásitos detectados. De 10 extractos de ADN de las diferentes especies de garrapatas positivos a piroplasmas; 5 extractos de ADN fueron de D. nitens, 3 de R. microplus y 2 de R. sanguineus, donde se identificaron mediante ITS1 B. canis vogeli, B. bigemina, B. equi (= T. equi) y B. caballi; mientras que por el gen 18S ARNr se identificó a B. equi, B. canis vogeli y B. caballi, inesperadamente a partir de un extracto de ADN de R. microplus se identificó Hepatozoon canis. La presente investigación constituye la evidencia de la gran diversidad de piroplasmas del género Babesia que se puede encontrar en especies de garrapatas de animales domésticos del departamento de Sucre. La alta diversidad de piroplasmas y las relaciones que se observaron con las especies de garrapatas en las que fueron detectadas, confirman la existencia de complejos patrones de interrelaciones garrapata-hospedero en zonas rurales del norte de Colombia que ameritan más estudios ecológicos y moleculares.Publicación Acceso abierto Distribución geográfica de especies de garrapatas infectadas con Babesia de zonas rurales del departamento de Sucre, Colombia.(Bogotá, Colombia : Memorias XXVI Simposio Internacional De Estadística, 2016., 2019-11-08) Guevara Vega, Marco; Vertel Morinson, Melba; Castellar Martínez, Anaís; Blanco Tuirán, PedroThe agents of the genus Babesia (Piroplasma: Babesiidae) are hemoparasites protozoa transmitted by ticks of the family Ixodidae during blood meal. In Colombia, the few studies of ticks, have focused on aspects of pest control in veterinary medicine and its impact on livestock, with very few contributions to the study of these ectoparasites as potential vectors of zoonotic diseases. In this paper, distribution models for tick species as R. (Boophilus) microplus, D. nitens, and R. sanguineus to estimate risk areas ixodológico in the Caribbean region were created. This distribution modeling was built by algorithms such as Maximum Entropy, and implemented in 3.3.3k Maxent program, taking the geographical points where there has been the presence of tick species and environmental variables to describe the ecological space used by each of them. All projections geographic distribution models generated were processed in QGIS 2.8.2 program. It was obtained as a result that some areas of the department of Cordoba can probably be these tick species and absent in places like the Sierra Nevada de Santa Marta. This is the first step in determining which areas are likely to find potential vectors of zoonotic agents of the genus Babesia.Publicación Acceso abierto Modelos potenciales de distribución geográfica y climática del complejo Amblyomma cajennense (Acari: Ixodidae), potencial vector de Rickettsia rickettsii en Colombia.(Bogotá, Colombia : Revista biomédica, 2018., 2019-09-23) Acevedo Gutiérrez, Leidy Y.; Paternina, Luis E.; Londoño, Andrés F.; Parra Henao, Gabriel; Rodas, Juan D.The species of the Amblyoma cajennense complex, potential vectors involved in the transmission of Rickettsia rickettsii in Central and South America, have a wide geographical distribution in the Neotropics. In Colombia, the knowledge about these species, their distribution, and many aspects of their ecology is still limited.