Examinando por Autor "Vélez, Iván Darío"
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Publicación Acceso abierto A new species of Sycorax (Diptera, Psychodidae, Sycoracinae) from the Pacific coast of Colombia.(Curitiba, Brasil : Revista Brasileira de Entomología, 2008., 2019-10-17) Bejarano, Eduar Elías; Duque, Patricia; Vélez, Iván Darío; Sociedade Brasileira de Entomología.A new species of Sycorax (Diptera, Psychodidae, Sycoracinae) from the Pacific coast of Colombia. Sycorax utriensis n. sp. is described and illustrated from a male specimen collected with a CDC light trap in the Parque Nacional Natural Ensenada de Utría, on the Pacific Coast of Colombia. Five species of Sycorax are now known from Colombia, S. andicola Young, 1979, S. colombiensis Young, 1979, S. fairchildi Young, 1979, S. trispinosa Young, 1979 and S. utriensis n. sp.Publicación Acceso abierto A new species of sycorax(Diptera, psychodidae, sycoracinae) from the pacific coast of Colombia(Curitiba: Sociedade Brasileira de Entomología,2008., 2019-09-16) Bejarano, Eduar Elías; Vélez, Iván Darío; Duque, PatriciaA new species of Sycorax (Diptera, Psychodidae,sycoracinae) from the pacific coast of Colombia.Sycorax utriensis n. sp. is described and illustrated from a male specimen collected with a CDC light trap in the parque Nacional Natural Ensenada de Utría,on the pacific Coast of Colombia.Five species of sycorax are now known from Colombia,S.andicola Young,1979,S.colombiensis Young,1979,S.trispinosa Young,1979 and S. utriensis n.sp.Publicación Acceso abierto Anomalías morfológicas en diferentes estructuras de cinco especies de Lutzomyia (Diptera: Psychodidae).(Buenos Aires, Argentina: Revista de la Sociedad Entomológica Argentina, 2008., 2019-10-21) Vergara, Daniela; Bejarano, Eduar Elías; Vélez, Iván Darío; Sociedad Entomológica Argentina.Diverse morphological anomalies in fi ve different species of Lutzomyia França (Diptera: Pychodidae) are described and illustrated. These theratologies are observed in various structures important for the taxonomic identifi cation of the species. The different individuals that belong to the species L. columbiana, L. hartmanni, L. reburra, L. ayrozai and L. panamensis were captured in diverse departments in Colombia.Publicación Acceso abierto Caracteres moleculares para la determinación taxonómica de tres especies de Lutzomyia (Diptera: Psychodidae), vectores potenciales de Leishmania presentes en el valle de Aburrá, Colombia.(Buenos aires, Argentina: Revista de la Sociedad Entomológica Argentina, 2008., 2019-10-18) Pérez Doria, Alveiro; Bejarano, Eduar Elías; Sierra, Diana; Vélez, Iván Darío; Sociedad Entomológica Argentina.To date, 143 species of Lutzomyia França are recorded in Colombia, but less than 7% is incriminated in the transmission of Leishmania spp. Alternative taxonomic characters are necessary to correctly identify the particular sand fl y fauna in each Colombian region, and the separation of morphologically similar vector and non-vector species. In order to detect useful molecular characters for the taxonomic determination of three potential vectors of Leishmania present in the Valle de Aburrá, Colombia, the present work sequenced the 3’ end of the mitochondrial cytochrome b gene in Lutzomyia hartmanni (Fairchild and Hertig), L. columbiana (Ristorcelli and Van Ty), and L. tihuiliensis Le Pont, Torrez-Espejo and Dujardin. Polymorphic sites, pairwise genetic distances (p), and entropy were determined from the multiple alignment of the nucleotide sequences. Numbers of silent and non silent substitutions were calculated from the amino acid sequences deduced from the nucleotide sequences of the gene. In the multiple alignment of the cytochrome b nucleotide sequences from Lutzomyia hartmanni, L. columbiana and L. tihuiliensis, 83 polymorphic sites were detected. A total of 18 amino acid replacements were found in the partial nucleotide sequences of the protein. Genetic distances varied from 0,137 between L. tihuiliensis and L. columbiana, to 0,215 among L. columbiana and L. hartmanni. Nucleotide and amino acid sequence polymorphisms within the cytochrome b gene and protein, respectively, constitute molecular characters potentially useful for the taxonomic determination of these sand fl y species.Publicación Acceso abierto Confirmación de la presencia de Lutzomyia Lutziana (Diptera: Psychodidae) en Colombia.(Bogotá, Colombia: Revista Caldasia, 2007., 2019-10-23) Bejarano, Eduar Elías; Duque, Patricia; Vélez, Iván DaríoThe presence of the sand fly Lutzomyia lutziana in Colombia is confirmed by one male specimen collected in the Serranía de La Macarena National Natural Park. This male was collected with a mouth aspirator, while resting in an armadillo nest. Lutzomyia lutziana resembles L. campograndensis, the former differing essentially by the slender tip of the genital filaments and the proportion of the length of the genital filaments and genital pump greater than 2,1.Publicación Acceso abierto Descripción del ARN de transferencia mitocondrial para Serina (UCN) de Lutzomyia columbiana (Diptera, Psychodidae).(Curitiba , Brasil: Revista Brasileira de Entomología, 2008., 2019-10-16) Pérez Doria, Alveiro; Bejarano, Eduar Elías; Vélez, Iván Darío; Sociedade Brasileira de Entomología.Description of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae). The sand fly Lutzomyia columbiana is considered a suspected vector of Leishmania mexicana and Leishmania braziliensis in Colombia. Lu. columbiana belongs to the Lutzomyia verrucarum species group, which included some sibling species. This has motivated the search for molecular markers to distinguish these taxa. In this paper, we described for the first time the putative secondary structure of the mitochondrial serine transfer RNA that recognizes the codon UCN of Lu. columbiana (tRNASer). DNA was extracted, amplified and sequenced from six individuals collected in human biting activity. The secondary structure of the tRNASer was inferred using the program tRNAscan-SE 1.21. The tRNASer gene length was 67 pair of bases (pb), and a single haplotype was detected among the six specimens sequenced. In the inferred secondary structure of the tRNASer of Lu. columbiana, the acceptor arm consisted of 7 bp, the dihydrouridine (DHU) arm of 3 pb, the anticodon arm of 5 pb, and the ribothymidine-pseudouridine-cytosine (TøC) arm of 5 pb. Similarity, the estimated size of the loops was 5 nucleotides in the DHU, 7 in the anticodon, 4 in the variable, and 7 in the TøC. Lu. columbiana differs from other Lutzomyia and Phlebotomus species sequenced to date by the presence of guanine in the nucleotide position 64, which induce a non-canonical base pair conformation type uracil-guanine in the acceptor arm. More studies are necessary to confirm the usefulness of the tRNASer as a suitable molecular tool for sand fly species identification.Publicación Acceso abierto Detección de anticuerpos anti-leishmania (trypanosomatidae) en poblaciones caninas del departamento de Sucre, Colombia.(Bogotá, Colombia: Acta biológica Colombiana, 2016., 2019-10-01) Paternina Tuirán, Luis Enrique; Díaz Olmos, Yiris Arleth; Paternina Gómez, Margaret; Carrillo Bonilla, Lina María; Vélez, Iván Darío; Bejarano, Eduar ElíasThe aim of this survey was to determine prevalence of antibodies against Leishmania in dogs from Sucre, Colombia. We analyzed 122 dogs in rural areas from municipalities of Sampués, Ovejas and Sincelejo, by indirect immunofluorescence (IFA). Anti-Leishmania antibodies were detected in 69.6 % (95 % CI = 61.1 to 78.2) of the selected canine population. In the municipality of Sincelejo, Sampues and Ovejas, the seropositivities were 72 % (95 % CI = 61.1 to 82.8), 68.4 % (95 % CI = 52.3 to 84.5) and 55.5 % (95 % CI = 21.2 to 86.3) respectively. Seropositive animals were mainly young and adult dogs. The 17.6 % of seropositive dogs showed clinical signs compatible with canine leishmaniasis; onychogryphosis, alopecia and cachexia were the most common ones. The detected high frequency of canines with antibodies against Leishmania in the present study, highlights the hyperendemicity status of canine leishmaniasis in this area of the country and the high risk of disease outbreaks.Publicación Acceso abierto Dos nuevos registros de Lutzomyia (Diptera: Psychodidae) para el Departamento de Risaralda, Colombia .(Bogotá, Colombia: Revista Colombiana de Entomología, 2007., 2019-10-22) Bejarano, Eduar Elías; Sierra, Diana; Vélez, Iván Darío; Sociedad Colombiana de Entomología .Two species of Lutzomyia sand flies are recorded for the first time in the department of Risaralda, Colombia: Lutzomyia atroclavata and Lutzomyia sp. series townsendi. The specimens of L. atroclavata were collected with a CDC light trap activated from 18:00 to 06:00 hours, whereas those of Lutzomyia serie townsendi were collected using human baits between 18:00 and 22:00 hours. The main diagnostic morphological characteristics of the species are described.Publicación Acceso abierto Estudio de los flebotomíneos (Diptera: Psychodidae) antropofílicos de la Serranía de La Macarena, Colombia.(Bogotá, Colombia: Revista Colombiana de Entomología, 2006., 2019-10-24) Bejarano, Eduar Elías; Duque, Patricia; Vélez, Iván Darío; Sociedad Colombiana de Entomología.A preliminary survey of the anthropophilic sand fly fauna of the Serranía de La Macarena, Colombia, is presented. Although leishmaniasis is endemic in the region, the vectors of the disease are yet unknown. Insects were collected in July and December using a mouth aspirator while they tried to bite humans between 18:00 and 22:00 hours. Ten species of Lutzomyia sand flies were identified from 89 female specimens collected: L. ayrozai, L. davisi, L. hirsuta hirsuta, L. carrerai carrerai, L. lichyi, L. amazonensis, L. sp. series squamiventris, L. yuilli, L. shawi, and L. sp. series townsendi. The subgenus Psychodopygus was most abundant, with six species that comprised 79.8% of all sand flies collected, followed by Nyssomyia with two species that constituted 16.9% of total captures. The verrucarum group and the subgenus Lutzomyia were each represented by one species. Finally, the epidemiological background of the species encountered are briefly reviewed.Publicación Acceso abierto Género Trichomyia Haliday, 1839 (Diptera: Psychodidae) asociado con Ficus Linn. (Moraceae) en la Amazonia Colombiana.(Manaus , Brasil: Acta Amazonica, 2009., 2019-10-15) Bejarano, Eduar Elías; Sierra, Diana; Vélez, Iván DaríoThe genus Trichomyia Haliday, 1839, is recorded for the first time in the Colombian Amazon, based on females collected in the municipality of Leticia, capital of the department of Amazonas. Psychodid flies were captured on sheets of paper impregnated with castor oil. The sticky traps were placed for seven days on trunks and tabular roots of Ficus Linn. trees at the edges of marsh habitats. A short taxonomic description of the collected specimens is included and the association between Trichomyia and this tree genus is discussedPublicación Acceso abierto Genetic analysis of a recently detected urban population of Lutzomyia evansi (Diptera: Psychodidae) in Colombia.(Buenos Aires, Argentina: Revista de la Sociedad Entomológica Argentina, 2009., 2019-10-16) Bejarano, Eduar Elías; Rojas, Winston; Uribe, Sandra; Vélez, Iván Darío; Porter, Cherles H.; Sociedad Entomológica Argentina.Lutzomyia evansi (Núñez-Tovar) is the vector of the parasite Leishmania infantum in rural zones of Northern Colombia. An attempt was made to determine the origin of a recently detected urban population of Lutzomyia evansi by genetically characterizing specimens from seven geographically distinct localities in the Colombian Caribbean. Insect specimens were collected in rural and urban environments of areas endemic for visceral leishmaniasis or free of the disease. Nine polymorphic sites, nine nucleotide haplotypes and a single aminoacid haplotype were found within the 315 bp fragment sequenced, corresponding to the 3’ end of the cytochrome b mitochondrial gene. Paired genetic distances between the haplotypes, estimated with the Kimura two-parameters model, varied from 0,0032-0,0194. Analysis revealed low genetic variability between specimens from urban and rural localities. Several of the sand flies collected in the city of Sincelejo (department of Sucre), where autochthonous visceral leishmaniasis cases have appeared in recent years, were genetically similar to those of a rural focus of the disease (El Contento, on the neighboring department of Córdoba). The epidemiological implications of this finding for Leishmania infantum transmission in the Colombian Caribbean are discussed.Publicación Acceso abierto Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.(Estados Unidos: Journal of Medical Entomology, 2008., 2019-11-08) Pérez Doria, Alveiro; Bejarano, Eduar Elías; Sierra, Diana; Vélez, Iván Darío; Entomological Society of America.The phlebotomine sand flies Lutzomyia pia (Fairchild & Hertig 1961) and Lutzomyia tihuiliensis Le Pont, Torrez-Espejo & Dujardin 1997 (Diptera: Psychodidae) belong to the pia series of the Lu. verrucarum species group, which includes several species that bite humans in Andean foci of leishmaniasis. The females of these two species exhibit isometry and isomorphism in anatomical structures of the head and terminalia commonly used in taxonomic identipcation of sand bies. They can only be differentiated based on subtle differences in the pigmentation of the pleura. In Lu. tihuiliensis, this is restricted to the basal portions of the katepimeron and katepisternum, whereas in Lu. piaboth structures are totally pigmented. Takinginto account the subtle morphological differences between these species, the objective of the current study was to evaluate the specipc taxonomic status of Lu. tihuiliensis with respect to Lu. pia. A 475-bp portion of the mitochondrial genome was sequenced, composed of the 3 end of the cytochrome b gene, intergenic spacer 1, the transfer RNA gene for serine, intergenic spacer 2, and the 3 end of the gene NAD dehydrogenase 1. Genetic analysis conÞrms that Lu. tihuiliensis and Lu. pia constitute two distinct species and this is supported by four strong lines of evidence, i.e., the paired genetic distances, size differences and amino acid composition of the cytochrome b protein, presence and absence of intergenic spacer one and divergence observed in the sequence of the transfer RNA gene for serine. It also conÞrms the validity of the pleural pigmentation pattern as a species diagnostic character and the importance of performing a detailed examination of this character during morphological determination of phlebotomine sand flies in the series pia.Publicación Acceso abierto Primer informe de Lutzomyia yuilli Young & Porter, 1972 y Lutzomyia triramula (Fairchild & Hertig 952) (Diptera: Psychodidae) en el departamento de Caldas, Colombia.(São Paulo, Brasil: Biota Neotropica , 2008., 2019-10-16) Vergara, Daniela; Carrillo, Lina María; Bejarano, Eduar Elías; Vélez, Iván DaríoNine species of Lutzomyia were collected during the research of various cutaneous leishmaniasis foci in the department of Caldas, Colombia: Lutzomyia gomezi (Nitzulescu, 1931), Lu. ayrozai (Barretto & Coutinho 1940), Lu. panamensis (Shannon 1926), Lu. bifoliata Osorno, Morales, Osorno & Hoyos, 1970, Lu. trapidoi (Fairchild & Hertig 1952), Lu. yuilli Young & Porter, 1972, Lu. triramula (Fairchild & Hertig 1952), Lu. atroclavata (Knab 1913), and Lu. trinidadensis (Newstead 1922). Two of these species constitute a new record for the department, Lu.yuilli of the subgenus Nyssomyia, and Lu. triramula of the subgenus Trichopygomyia. The specimens of Lu. yuilli were collected using Shannon and CDC light traps in the municipality of Norcasia, while the specimens of Lu. triramula were collected using light and sticky traps in both Norcasia and Victoria municipalities. The importance of this finding, taking into account the limited distribution of Lu. triramula in the Colombian territory and the vector capacity of Lu. yuilli is considered.Publicación Acceso abierto Primer registro de Lutzomyia scorzai y Lutzomyia reburra (Diptera: Psychodidae) en Antioquia, Colombia.(Bogotá, Colombia: Revista Colombiana de Entomologia, 2008., 2019-10-07) Vergara, Daniela; Bejarano, Eduar Elías; Carrillo, Lina María; Sierra, Diana; Vélez, Iván Darío; Sociedad Colombiana de Entomología.Seven species of Lutzomyia and one of Brumptomyia were collected while surveying two cutaneous leishmaniosis foci in the department Antioquia, Colombia, these are: Lutzomyia gomezi, Lu. scorzai, Lu. trapidoi, Lu. triramula, Lu. panamensis, Lu. aragaoi, Lu. reburra and Brumptomyia galindoi. Lu. scorzai (subgenus Helcocyrtomyia) and Lu. reburra (subgenus Trichophoromyia) are new records for the department. The specimens of Lu. scorzai were captured using a Shannon light trap in the municipality of Tarazá, while those of Lu. reburra were collected using CDC-like light traps near a nest of armadillo in the municipality of Urrao. The biogeographic importance of these findings is discussed given the limited distribution of both species in Colombia.Publicación Acceso abierto Primer registro del flebotomíneo Brumptomyia pintoi (Diptera: Psychodidae) en Colombia.(Bogotá, Colombia: Revista Caldasia , 2004., 2019-10-25) Bejarano, Eduar Elías; Duque, Patricia; Vélez, Iván DaríoOne male of Brumptomyia pintoi was collected during an entomological survey of leishmaniasis conducted in La Macarena, Department of Meta, Colombia. This is the first record of B. pintoi from this country. The sand fly was captured from an armadillo burrow with a sucking tube.Publicación Acceso abierto Redescripción de la hembra de Lutzomyia vattierae (Diptera: Psychodidae, Phlebotominae) de la serranía de La Macarena, Colombia.(Bogotá, Colombia: Revista Biomédica, 2006., 2019-10-24) Bejarano, Eduar Elías; Duque, Patricia; Vélez, Iván DaríoLutzomyia species of the subgenus Sciopemyia show restricted distribution andare rarely represented in sand fly captures, with the exception of L. sordellii, which is found fromCosta Rica to Argentina. To date, only two of these species. L. sordellii and L. preclara havebeen reported in Colombia. The female of L. vattierae was redescribedand the record of this Sciopemyia speciesestablished for in the Colombian national park, serranía de La Macarena.Publicación Acceso abierto Trece registros nuevos de Lutzomyia (Diptera: Psychodidae) para el departamento de Vichada, Orinoquia Colombiana.(São Paulo, Brasil : Biota Neotropica, 2010., 2019-10-11) Vivero, Rafael José; Bejarano, Eduar Elías; Castro, Mirley; Vélez, Andrés; Pérez, Juan Esteban; Pérez Doria, Alveiro; Vélez, Iván DaríoLutzomyia trinidadensis (Newstead, 1922), L. bettinii Feliciangeli, Ramírez-Pérez & Ramírez, 1988, L. flaviscutellata (Mangabeira, 1942), L. yuilli Young & Porter, 1972, L. saulensis (Floch & Abonnenc, 1944), L. runoides (Fairchild & Hertig, 1953), L. ayrozai (Barretto & Coutinho, 1940), L. claustrei Abonnenc, Leger & Fauran, 1979, L. walkeri (Newstead, 1914), L. preclara Young & Arias, 1984, L. (geniculata) sp., L. (squamiventris) sp., and L. (Evandromyia) sp., are recorded for the first time in the department of Vichada, Colombia. In addition, the presence of L. antunesi (Coutinho, 1939) and L. aragaoi (Costa Lima, 1932) in this department is confirmed. Sand flies were collected in the municipalities of Puerto Carreño, Cumaribo, and La Primavera, using CDC light traps, Shannon trap, and human bait, and by active search in resting places. The epidemiological importance of these sand fly species is discussed in relation with the cutaneous leishmaniasis.