Examinando por Autor "Paternina, Luis E."
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Publicación Acceso abierto Actualización de la distribución geográfica de especies de Leishmania, caracterizadas por genética molecular, en el macrofoco de leishmaniasis del Caribe colombiano.(Bogotá, Colombia: Revista Biomédica, 2011., 2019-10-28) Martínez, Lily; Paternina Gómez, Margaret; Paternina, Luis E.; Pérez Doria, Alveiro; Bejarano, Eduar E.En Colombia existe un limitado conocimiento sobre la distribución geográfica de las especies que causan leishmaniasis, lo cual se debe a que el diagnóstico de la enfermedad y la instauración del tratamiento se basa casi exclusivamente en el examen microscópico directo, sin precisar la especie de Leishmania causal, a pesar de que existe una susceptibilidad diferencial del parásito a los medicamentos empleados. En este trabajo se actualiza la distribución geográfica de las especies de Leishmania en el norte de Colombia.Publicación Acceso abierto An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses.(Estados Unidos : María S. Salvato, 2018., 2019-11-22) Díaz, Francisco J.; Paternina, Luis E.; Rodas, Juan DavidAn important aspect of virological surveillance is the identification of the detected viruses. Broad surveillance, that typically employs deep sequencing of collected tissue samples, provides the investigator with many sequence files constructed from overlapping stretches of DNA sequences. Directed surveillance for viruses of a specific taxonomic group provides the investigator with sequence files from cDNA amplified using specific primers to conserved viral regions. Here we will describe general approaches to identify hemorrhagic viral agents through phylogenetic analysis of cDNA sequences obtained during surveillance activities.Publicación Acceso abierto Canis familiaris, un nuevo hospedero de Ornithodoros (A.) puertoricensis FOX, 1947 (Acari: Ixodida) en Colombia.(Bogotá, Colombia: Acta Biológica Colombiana , 2009., 2019-10-15) Paternina, Luis E.; Díaz Olmos, Yirys; Paternina Gómez, Margaret; Eduar E., BejaranoTicks are very important from the biomedical point of view, by their hematophagic activity and their role in the transmission of pathogenic microorganisms to man and animals. The main goal of this work was to establish the tick species parasiting dogs in three rural localities of the Colombian Caribbean. From August to December 2006, an active search of ticks on dogs was carried out in the localities of El Campín, Sabanas del Potrero and Escobar Arriba, department of Sucre. The collected ticks were preserved into eppendorf tubes with 70% ethanol, and identified using standard morphological keys for each family. Argasid species were determined by measuring external morphological characters. Of 134 examined dogs in the three localities, 50 were found infested by ticks, representing a infestation rate of 37,3%. A total of 420 ticks were collected from dogs and identified as Rhipicephalus sanguineus, Rhipicephalus (Boophilus) microplus, and Amblyomma ovale of the Ixodidae family, and Ornithodoros puertoricensis of the Argasidae family. R. sanguineus was the predominant species (92,1%) in the stages of larva, nymph and adult, following by O. puertoricensis larvae recorded in low numbers in the three regions sampled. The tick O. puertoricensis is recorded for the first time as ectoparasite of domestic dogs in America. Additionally, the presence of this tick species is confirmed in Colombia.Publicación Acceso abierto Comparación y utilidad de tres métodos de extracción de ADN a partir de potenciales vectores de enfermedades rickettsiales (Acari: Ixodidae) en Colombia.(Bogotá, Colombia: Memorias XXV Simposio Internacional De Estadística, 2015., 2019-11-08) Guevara Vega, Marco; Paternina, Luis E.; Blanco Tuiran, Pedro; Vertel Morinson, MelbaThe study of diseases transmitted for tick as rickettsia is of great interest to medical and veterinary level and requires the use of extracts of good quality DNA for subsequent analysis of amplified genes of these pathogens. In this study, three methods were compared DNA extraction from ticks complex Rhipicephalus sanguineus based on: chaotropic guanidine thiocyanate (method 1), high salt (method 2) and an extraction method based on commercially available columns (Method 3). The DNA was quantified and the quality of each extract in the amplification of the 16S gene ticks (360pb) was evaluated, a nonparametric Kruskal-Wallis one-way analysis was performed, while comparing the amplification efficiency of mitochondrial gene was performed by Dunn test range. Method 3 allowed the amplification of the 16S gene of ticks in 100% (30/30) followed by method 1 with 93.33% (28/30) and finally with method 2 86.66% (26/30). The three methods are efficient at extracting genetic material of high quality tick-borne studies since their performance in amplification of mitochondrial genes tick agents.Publicación Acceso abierto Distribution of Spotted Fever Group Rickettsiae in Hard Ticks (Ixodida: Ixodidae) from Panamanian Urban and Rural Environments (2007-2013).(Estados Unidos: Ecohealth, 2016., 2019-10-05) Bermúdez, Sergio E.; Castro, Angélica M.; Trejos, Diomedes; García, Gleydis G.; Gabster, Amanda; Miranda, Roberto J.; Zaldívar, Yamitzel; Paternina, Luis E.Tick-borne rickettsiosis is an important emerging disease in Panama; to date, there have been 12 confirmed cases, including eight fatalities. To evaluate the distribution of rickettsiae in Panamanian ticks, we collected questing and on-host ticks in urban and rural towns in elevations varying between 0 and 2300 m. A total of 63 sites (13 urban and 50 rural towns) were used to develop models of spatial distributions. We found the following tick species: Rhipicephalus sanguineus s.l. (present in 54 of 63 towns and cities), Amblyomma mixtum (45/63), Dermacentor nitens (40/63), A. ovale (37/63), Rhipicephalus microplus (33/63), A. oblongoguttatum (33/63), Ixodes affinis (3/63), and Ixodes boliviensis (2/63). Rhipicephalus sanguineus s.l. was present in urban and rural towns, and other species were present only in rural towns. DNA was extracted from 408 R. sanguineus s.l., 387 A. mixtum, 103 A. ovale, and 11 A. oblongoguttatum and later tested for rickettsiae genes using PCR. Rickettsia DNA was detected in ticks from 21 of 63 localities. Rickettsia rickettsii was detected in five A. mixtum (1.29%), and Candidatus ‘‘Rickettsia amblyommii’’ was found in 138 A. mixtum (35%), 14 R. sanguineus (3.4%), and one A. ovale (0.9%). These results suggest that much of rural Panama is suitable for the expansion of tick populations and could favor the appearance of new tick-borne rickettsiosis outbreaks.Publicación Acceso abierto Modelos potenciales de distribución geográfica y climática del complejo Amblyomma cajennense (Acari: Ixodidae), potencial vector de Rickettsia rickettsii en Colombia.(Bogotá, Colombia : Revista biomédica, 2018., 2019-09-23) Acevedo Gutiérrez, Leidy Y.; Paternina, Luis E.; Londoño, Andrés F.; Parra Henao, Gabriel; Rodas, Juan D.The species of the Amblyoma cajennense complex, potential vectors involved in the transmission of Rickettsia rickettsii in Central and South America, have a wide geographical distribution in the Neotropics. In Colombia, the knowledge about these species, their distribution, and many aspects of their ecology is still limited.Publicación Acceso abierto Seroprevalencia a Rickettsia del grupo de las fiebres manchadas, en población humana de zona rural del municipio de Toluviejo, Colombia.(Sincelejo, Colombia: Revista De Investigaciones En Medicina Tropical, 2015., 2019-11-12) Arrieta Hernández, Norma; Salgado Mercado, Andrés; Paternina, Luis E.; Martínez, Lily; Vertel Morinson, Melba; Paternina Gómez, Margaret; Bejarano, Eduar E.Rickettsia is a genus consisting of obligate intracellular bacteria; the main vectors are arthropod ectoparasites, espe-cially ticks. In 2008, was detected in Toluviejo, Colombia, a seropositive person to Rickettsia, however, there is little information about the prevalence in this municipality.Objetives. To study the seroprevalence of Rickettsia of the spotted fever group and it’s relation with socio-demographic factors in a human population in Toluviejo, Colombia.