Examinando por Autor "Paternina, Luís E."
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Publicación Acceso abierto Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.(Reino Unido: Acta Tropica, 2015., 2019-11-06) Paternina, Luís E.; Verbel Vergara, Daniel; Romero Ricardo, Luís; Pérez Doria, Alveiro; Paternina Gómez, Margaret; Martínez, Lily; Bejarano, Eduar E.Identification of the bloodmeal sources of phlebotomine sand flies is fundamental to determining which species are anthropophilic and understanding the transmission of Leishmania parasites in natural epidemiological settings. The objective of this study was to identify sand fly bloodmeals in the mixed leishmaniasis focus of the department of Sucre, northern Colombia. In all 141 engorged female sand flies were analyzed, after being captured in intradomiciliary, peridomiciliary and extradomiciliary habitats with Shannon and CDC traps and by active searching in diurnal resting sites. Bloodmeals were identified by sequencing and analysis of a 358 bp fragment of the mitochondrial gene Cytochrome b (CYB) and a 330 bp fragment of the nuclear gene prepronociceptin (PNOC). Using both genes 105 vertebrate bloodmeals were identified, with an efficiency of 72% for CYB but only 7% for PNOC. Ten species of vertebrates were identified as providing bloodmeal sources for eight sand fly species: Homo sapiens (Lutzomyia evansi, Lu. panamensis, Lu. micropyga, Lu. shannoni and Lu. atroclavata), Equus caballus (Lu. evansi, Lu. panamensis and Lu. cayennensis cayennensis), Eq. asinus (Lu. evansi and Lu. panamensis), Bos taurus 4 (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Tamandua mexicana (Lu. shannoni and Lu. trinidadensis), Proechimys guyanensis (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Mabuya sp. (Lu. micropyga), Sus scrofa (Lu. evansi and Lu. gomezi) and Gallus gallus (Lu. evansi). Cattle, donkeys, humans and pigs were significantly more important than other animals (P = 0.0001) as hosts of Lu. evansi, this being the most abundant sand fly species. The five Lutzomyia species in which blood samples of human origin were detected included Lu. micropyga and Lu. atroclavata, constituting the first evidence of anthropophily in both species.Publicación Acceso abierto First report of Amblyomma dissimile (Acari: Ixodidae) on Spilotes pullatus (Squamata: Colubridae) from Colombia.(Sincelejo, Colombia: Revista De Investigaciones En Medicina Tropical, 2015., 2019-11-08) Verbel Vergara, Daniel E.; Bejarano, Eduar E.; Paternina, Luís E.The very few studies about the tick fauna of Colombia have focused on ectoparasites of domestic and farm animals, therefore little information about the tick fauna in wildlife is available. The purpose of this research is to contribute to the knowledge of ticks that parasitize snakes in Colombia.Publicación Acceso abierto Sampling Design and Mosquito Trapping For Surveillance of Arboviral Activity.(India: Maria S. Salvato, 2017., 2019-11-22) Paternina, Luís E.; Rodas, Juan DavidMosquitoes are the most important vectors for arboviral human diseases across the world. Diseases such as Dengue Fever (DF), West Nile Virus (WNV), Yellow Fever (YF), Japanese Encephalitis (JE), Venezuelan Equine Encephalitis (VEE), and St. Louis Encephalitis (SLE), among others, have a deep impact in public health. Usually mosquitoes acquire the arboviral infection when they feed on viremic animals (birds or mammals), so their infection can be detected along the year or in short periods of time (seasons). All of this depends on the frequency and seasonality of the encounters between viremic animals and vectors. With the convergence of several phenomena like the increasing traveling of human populations, globalization of economy and more recently the global warming, the introduction of nonendemic arbovirus into new areas has become the current scenario. As examples of this new social and environmental frame we can mention the outbreak of West Nile Virus in North America in the late 1990s and more recently the outbreaks of chikungunya and Zika virus in the Americas. The present chapter deals with one of the first steps in the development of research studies and diagnosis programs, the surveillance of arboviruses in their vectors, the sampling design and mosquito trapping methods. The chapter also includes some important considerations and tips to be taken into account during the mosquito fieldwork.