Examinando por Autor "Pérez Doria, Alveiro"
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Publicación Acceso abierto Actualización de la distribución geográfica de especies de Leishmania, caracterizadas por genética molecular, en el macrofoco de leishmaniasis del Caribe colombiano.(Bogotá, Colombia: Revista Biomédica, 2011., 2019-10-28) Martínez, Lily; Paternina Gómez, Margaret; Paternina, Luis E.; Pérez Doria, Alveiro; Bejarano, Eduar E.En Colombia existe un limitado conocimiento sobre la distribución geográfica de las especies que causan leishmaniasis, lo cual se debe a que el diagnóstico de la enfermedad y la instauración del tratamiento se basa casi exclusivamente en el examen microscópico directo, sin precisar la especie de Leishmania causal, a pesar de que existe una susceptibilidad diferencial del parásito a los medicamentos empleados. En este trabajo se actualiza la distribución geográfica de las especies de Leishmania en el norte de Colombia.Publicación Acceso abierto Aislamiento de Leishmania infantum a partir de Canis familiaris en área urbana del Caribe colombiano(Lima, Perú: Revista de Investigaciones Veterinarias del Perú, 2018, 2019-09-20) Rivero Rodríguez, Matilde Elena; Rodríguez Jiménez, Jorge Luis; Pérez Doria, Alveiro; Bejarano, Eduar ElíasThe isolation of parasites of the genus Leishmania is reported from a popliteal ganglion biopsy obtained from a dog with canine visceral leishmaniasis. The dog lived in the urban area of the municipality of Ovejas, Sucre, Colombia. The Indirect Immunofluorescence test was positive for anti-Leishmania spp antibodies up to a dilution of 1/128. The typing of L. infantum was performed by isoenzyme electrophoresis and by 924 Rev Inv Vet Perú 2018; 29(3): 923-930 M. Rivero et al. analysis of the sequence of the cytochrome b gene and the nuclear gene that codes for the heat shock protein of 70 Kda (Hsp70). The present finding is the first confirmation of infection by L. infantum in canines in the urban area of the Colombian Caribbean.Publicación Acceso abierto Brumptomyia hamata (Psychodidae), un nuevo aporte a la fauna flebotomínea del Caribe colombiano.(Bogotá, Colombia: Acta Biológica Colombiana, 2009., 2019-10-11) Pérez Doria, Alveiro; Hernández Oviedo, Ela; Bejarano, Eduar ElíasThe sand fly fauna of the Colombian Caribbean is still not completely known and furthersurveys are needed in order to determine the richness of species over the region.Entomological samplings were performed in the municipality of Colosó, departmentof Sucre, using two modified CDC traps, in January and March of 2007. Two malesbelonging to Brumptomyia hamatawere identified. It is the first record of this sand flyspecies from the Colombian Caribbean. With the exception of the distribution of spineson the style in three distinct groups, all other morphological characters of the individualsof B. hamataagree with the original description of the species. The observed differencecould be attributed to intraspecific variation among geographic populations.Publicación Acceso abierto Caracteres moleculares para la determinación taxonómica de tres especies de Lutzomyia (Diptera: Psychodidae), vectores potenciales de Leishmania presentes en el valle de Aburrá, Colombia.(Buenos aires, Argentina: Revista de la Sociedad Entomológica Argentina, 2008., 2019-10-18) Pérez Doria, Alveiro; Bejarano, Eduar Elías; Sierra, Diana; Vélez, Iván Darío; Sociedad Entomológica Argentina.To date, 143 species of Lutzomyia França are recorded in Colombia, but less than 7% is incriminated in the transmission of Leishmania spp. Alternative taxonomic characters are necessary to correctly identify the particular sand fl y fauna in each Colombian region, and the separation of morphologically similar vector and non-vector species. In order to detect useful molecular characters for the taxonomic determination of three potential vectors of Leishmania present in the Valle de Aburrá, Colombia, the present work sequenced the 3’ end of the mitochondrial cytochrome b gene in Lutzomyia hartmanni (Fairchild and Hertig), L. columbiana (Ristorcelli and Van Ty), and L. tihuiliensis Le Pont, Torrez-Espejo and Dujardin. Polymorphic sites, pairwise genetic distances (p), and entropy were determined from the multiple alignment of the nucleotide sequences. Numbers of silent and non silent substitutions were calculated from the amino acid sequences deduced from the nucleotide sequences of the gene. In the multiple alignment of the cytochrome b nucleotide sequences from Lutzomyia hartmanni, L. columbiana and L. tihuiliensis, 83 polymorphic sites were detected. A total of 18 amino acid replacements were found in the partial nucleotide sequences of the protein. Genetic distances varied from 0,137 between L. tihuiliensis and L. columbiana, to 0,215 among L. columbiana and L. hartmanni. Nucleotide and amino acid sequence polymorphisms within the cytochrome b gene and protein, respectively, constitute molecular characters potentially useful for the taxonomic determination of these sand fl y species.Publicación Acceso abierto Descripción de una nueva especie de Trichomyia (Diptera: Psychodidae) de los Andes Colombianos.(Buenos Aires, Argentina: Revista de la Sociedad Entomológica Argentina, 2009., 2019-10-15) Bejarano, Eduar Elías; Pérez Doria, Alveiro; Sierra, Diana; Sociedad Entomológica Argentina.Trichomyia risaraldensis sp. nov., a new species of Psychodidae from the Colombian Andes, is described and illustrated. The type material was collected using a CDC light trap in the municipality of Marsella, department of Risaralda, Colombia. Morphologically speaking, Trichomyia risaraldensis sp. nov. resembles Trichomyia amata Barretto, Trichomyia brasiliensis Satchell, Trichomyia pseudodactylis Quate, and Trichomyia quatei Bravo, but can be distinguished from those species by traits of the head, wing and male genitalia. With the description of Trichomyia risaraldensis sp. nov., the number of Neotropical species in the genus Trichomyia is increased to 71.Publicación Acceso abierto Descripción del ARN de transferencia mitocondrial para Serina (UCN) de Lutzomyia columbiana (Diptera, Psychodidae).(Curitiba , Brasil: Revista Brasileira de Entomología, 2008., 2019-10-16) Pérez Doria, Alveiro; Bejarano, Eduar Elías; Vélez, Iván Darío; Sociedade Brasileira de Entomología.Description of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae). The sand fly Lutzomyia columbiana is considered a suspected vector of Leishmania mexicana and Leishmania braziliensis in Colombia. Lu. columbiana belongs to the Lutzomyia verrucarum species group, which included some sibling species. This has motivated the search for molecular markers to distinguish these taxa. In this paper, we described for the first time the putative secondary structure of the mitochondrial serine transfer RNA that recognizes the codon UCN of Lu. columbiana (tRNASer). DNA was extracted, amplified and sequenced from six individuals collected in human biting activity. The secondary structure of the tRNASer was inferred using the program tRNAscan-SE 1.21. The tRNASer gene length was 67 pair of bases (pb), and a single haplotype was detected among the six specimens sequenced. In the inferred secondary structure of the tRNASer of Lu. columbiana, the acceptor arm consisted of 7 bp, the dihydrouridine (DHU) arm of 3 pb, the anticodon arm of 5 pb, and the ribothymidine-pseudouridine-cytosine (TøC) arm of 5 pb. Similarity, the estimated size of the loops was 5 nucleotides in the DHU, 7 in the anticodon, 4 in the variable, and 7 in the TøC. Lu. columbiana differs from other Lutzomyia and Phlebotomus species sequenced to date by the presence of guanine in the nucleotide position 64, which induce a non-canonical base pair conformation type uracil-guanine in the acceptor arm. More studies are necessary to confirm the usefulness of the tRNASer as a suitable molecular tool for sand fly species identification.Publicación Acceso abierto Diferencias genéticas entre poblaciones de Aedes aegypti de municipios del norte de Colombia, con baja y alta incidencia de dengue.(Bogotá, Colombia: Revista Biomédica, 2013., 2019-10-04) Caldera, Sandy Milena; Jaramillo, María Cristina; Cochero, Suljey; Pérez Doria, Alveiro; Bejarano, Eduar ElíasAedes aegypti is the principal vector of dengue in urban areas. Despite its epidemiological importance, the genetic variability of Colombian populations of this species is unknown. To determine the genetic variability of mitocondrial gene ND4, which codes for subunit 4 of the enzyme NADH deshydrogenase, between populations of Ae. aegypti from municipalities of Sincelejo and Guaranda. The incidences of dengue reported from these two localities are high and low, respectively.Publicación Acceso abierto DNA barcoding to identify species of phlebotomine sand fly (Diptera: Psychodidae) in the mixed leishmaniasis focus of the Colombian Caribbean.(Países Bajos: Revista Acta Tropica, 2016., 2019-09-27) Romero Ricardo, Luis; Lastre Meza, Natalia; Bejarano, Eduar E.; Pérez Doria, AlveiroIdentification of the species of phlebotomine sand flies present in each focus of leishmaniasis is necessary to incriminate vectors and implement vector control strategies. Although the cytochrome oxidase I (COI) gene has been proposed as a barcode for the identification of animal species, less than 20% of New World phlebotomines have been characterized to date. In this study DNA barcoding was used to identify phlebotomine species of the mixed leishmaniasis focus in the Colombian Caribbean by means of three evolutionary models: Kimura’s two parameter (K2P) nucleotide substitution model, that of (Tamura and Nei, 1993) (TN93) and proportional sequence divergence (p-distances). A 681 bp sequence of the COI gene was obtained from 66 individuals belonging to 19 species of the genus Lutzomyia (Lu. abonnenci, Lu. atroclavata, Lu. bicolor, Lu. carpenteri, Lu. cayennensis cayennensis, Lu. dubitans, Lu. evansi, Lu. gomezi, Lu. gorbitzi, Lu. longipalpis, Lu. micropyga, Lu. migonei, Lu. panamensis, Lu. (Psathyromyia) sp., Lu. rangeliana, Lu. serrana, Lu. shannoni, Lu. trinidadensis and Lu. venezuelensis) and one of Brumptomyia (B. mesai).Publicación Acceso abierto Estimación del tiempo límite de detección del gen citocromo b de humanos en hembras de Lutzomyia evansi.(Bogotá, Colombia : Revista biomédica, 2017., 2019-09-25) Vergara, José Gabriel; Verbel Vergara, Daniel; Bejarano, Eduar Elías; Montesino, Ana Milena; Pérez Doria, AlveiroMolecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect.Publicación Acceso abierto Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.(Reino Unido: Acta Tropica, 2015., 2019-11-06) Paternina, Luís E.; Verbel Vergara, Daniel; Romero Ricardo, Luís; Pérez Doria, Alveiro; Paternina Gómez, Margaret; Martínez, Lily; Bejarano, Eduar E.Identification of the bloodmeal sources of phlebotomine sand flies is fundamental to determining which species are anthropophilic and understanding the transmission of Leishmania parasites in natural epidemiological settings. The objective of this study was to identify sand fly bloodmeals in the mixed leishmaniasis focus of the department of Sucre, northern Colombia. In all 141 engorged female sand flies were analyzed, after being captured in intradomiciliary, peridomiciliary and extradomiciliary habitats with Shannon and CDC traps and by active searching in diurnal resting sites. Bloodmeals were identified by sequencing and analysis of a 358 bp fragment of the mitochondrial gene Cytochrome b (CYB) and a 330 bp fragment of the nuclear gene prepronociceptin (PNOC). Using both genes 105 vertebrate bloodmeals were identified, with an efficiency of 72% for CYB but only 7% for PNOC. Ten species of vertebrates were identified as providing bloodmeal sources for eight sand fly species: Homo sapiens (Lutzomyia evansi, Lu. panamensis, Lu. micropyga, Lu. shannoni and Lu. atroclavata), Equus caballus (Lu. evansi, Lu. panamensis and Lu. cayennensis cayennensis), Eq. asinus (Lu. evansi and Lu. panamensis), Bos taurus 4 (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Tamandua mexicana (Lu. shannoni and Lu. trinidadensis), Proechimys guyanensis (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Mabuya sp. (Lu. micropyga), Sus scrofa (Lu. evansi and Lu. gomezi) and Gallus gallus (Lu. evansi). Cattle, donkeys, humans and pigs were significantly more important than other animals (P = 0.0001) as hosts of Lu. evansi, this being the most abundant sand fly species. The five Lutzomyia species in which blood samples of human origin were detected included Lu. micropyga and Lu. atroclavata, constituting the first evidence of anthropophily in both species.Publicación Acceso abierto Flebotomíneos (Diptera: Psychodidae) antropofílicos de importancia en salud pública en Los Montes de María, Colombia.(La Habana , Cuba: Revista Cubana de Medicina Tropical, 2009., 2019-10-15) Cortés Alemán, Luis; Pérez Doria, Alveiro; Bejarano Martínez, Eduar ElíasLa leishmaniasis cutánea es una enfermedad endémica en Los Montes de María, Colombia, pero hasta la fecha se desconocían los flebotomíneos que pican al humano en el área. Los estudios previos realizados en esta zona se han orientado hacia la caracterización de los insectos que actúan como vectores en el ciclo epidemiológico de la leishmaniasis visceral, 6,12-17 porque esta última puede tener una evolución fatal. El objetivo de esta investigación fue determinar las especies de Lutzomyia que, por sus hábitos antropofílicos y antecedentes vectoriales, podrían desempeñarse como transmisores en el ciclo epidemiológico de la leishmaniasis cutánea en el área rural de Los Montes de María.Publicación Acceso abierto Identificación morfológica y molecular de especies del género haemagogus (diptera: culicidae) en la región caribe colombiana.(2017) Cochero Bustamante, Suljey; Pérez Doria, AlveiroLos mosquitos del género Haemagogus son importantes en salud pública, por ser vectores del virus Fiebre Amarilla y del virus Mayaro. Este trabajo permitió identificar seis especies de este género en tres departamentos de Colombia: Sucre, Atlántico y Cesar, basado en caracteres morfológicos y moleculares mediante la amplificación de un fragmento de los genes Citocromo Oxidasa I y White, utilizando las técnicas de PCR y secuenciación. Los mosquitos se recolectaron a través de larvitrampas, búsqueda directa en criaderos potenciales y captura adultos. La determinación taxonómica se realizó con las claves y descripciones de Arnell (1973) y Liria y Navarro (2009). Se capturaron 8863 estados inmaduros de mosquitos agrupados en seis géneros: Haemagogus (77%), Aedes (13%), Limatus (6%), Culex (4%), Toxorhynchites (0,34%) y Wyeomyia (0,13%). El género Haemagogus en el departamento de Sucre estuvo representado por 6 especies: H. lucifer, H. janthinomys, H. equinus, H. celeste, H. anastasionis y H. chalcospilans; dos especies fueron capturadas en los departamentos de Atlántico y Cesar: H. equinus y H. anastasionis. La presencia de H. janthinomys, H. equinus y H. lucifer constituyen un riesgo en la transmisión de fiebre amarilla en estos departamentos. Los análisis realizados a partir de un fragmento de 617 pb de la secuencia del gen mitocondrial COI demuestran su utilidad en la discriminación de seis especies del género Haemagogus y en la realización de inferencias filogenéticas. Los árboles obtenidos agrupan las especies en dos clados, resultados consistentes con la taxonomía clásica propuesta por Arnell (1973) para dos secciones de este género: Albomaculatus y Splendens; el análisis de un fragmento de 223 pb de las secuencias del gen White solo pudo discriminar tres especies: H. lucifer, H. celeste y H. chalcospilans y careció de resolución para diferenciar especies estrechamente relacionadas como H. equinus, H. anastasionis y H. janthinomys. Este estudio confirma la utilidad de las técnicas de biología molecular como apoyo a técnicas morfológicas tradicionales para la discriminación de especies de mosquitos.Publicación Acceso abierto Lutzomyia (Diptera: Psychodidae) de la Reserva Serranía de Coraza y Montes de María, Colombia.(Bogotá, Colombia: Revista Colombiana de Entomología, 2008., 2019-10-21) Pérez Doria, Alveiro; Hernández Oviedo, Ela; Bejarano, Eduar Elías; Sociedad Colombiana de Entomología.The number of species of the genus Lutzomyia found in the North coast of Colombia is relatively small compared to the diversity observed in other biogeographic zones of the country. In part, this situation may be attributed to the many unexplored areas that remain in the region. The goal of this study was to examine the diversity of the genus Lutzomyia in the Reserva Forestal Protectora Serranía de Coraza y Montes de María, department of Sucre. Insects were collected in April and June 2006, by using a modified CDC light trap operated between 18:00 and 06:00 hours. A total of 3.335 specimens were obtained belonging to eight species, including L. evansi, L. panamensis, L. longipalpis, L. gomezi, L. trinidadensis, L. dubitans, L. carpenteri and L. camposi. Four medically important species were the most abundant, L. evansi with 97.24% of the sand flies found, followed by L. panamensis with 2.01%, L. longipalpis with 0.33%, and L. gomezi with 0.27%. The presence of L. carpenteri and L. camposi is discussed from the taxonomic and biogeographic viewpoint as the first representatives of the Lutzomyia aragaoi group and the subgenus Pressatia, respectively, found in the Colombian Atlantic coast.Publicación Acceso abierto Lutzomyia abonneci y Lutzomyia olmeca bicolor (Diptera: Psychodidae), nuevos registros para el departamento de Sucre, Colombia.(Bogotá, Colombia: Acta Biológica Colombiana , 2013., 2019-10-03) Romero Ricardo, Luis; Lastre Meza, Natalia; Pérez Doria, Alveiro; Bejarano Martínez, EduarAmong Colombia’s Caribbean coast departments, Sucre has the greatest species diversity of phlebotomine sand flies (genus Lutzomyia), including several associated with the epidemiological cycles of cutaneous and visceral leishmaniasis. Due to its public health importance, the phlebotomine fauna of Sucre must be monitored periodically. Sand flies were collected in the ecological reserve of the municipality of Colosó, Sucre, during the months of December 2009, 2010 and 2011. Three sampling methods were employed, i.e., daytime collections from diurnal resting sites with a battery-powered aspirator and nocturnal collections using two CDC light traps and a Shannon trap. With these methods 566 phlebotomines were collected belonging to 14 species: Lu.evansi, Lu. panamensis, Lu. micropyga, Lu.gomezi, Lu. cayennensis cayennensis, Lu. trinidadensis, Lu.venezuelensis, Lu. serrana, Lu. abonnenci, Lu. migonei, Lu. dubitans, Lu. olmeca bicolor, Lu. atroclavata and Lu. ovallesi. Shannon trap collections accounted for 48,2 % of all phlebotomines collected, those with CDC light traps 36,6 % and with battery-powered aspirator 15,2 %. Both Lu. abonnenci and Lu. o. bicolor, species with wide spread in Colombia, represent new species records for the department of Sucre.Publicación Acceso abierto Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.(Estados Unidos: Journal of Medical Entomology, 2008., 2019-11-08) Pérez Doria, Alveiro; Bejarano, Eduar Elías; Sierra, Diana; Vélez, Iván Darío; Entomological Society of America.The phlebotomine sand flies Lutzomyia pia (Fairchild & Hertig 1961) and Lutzomyia tihuiliensis Le Pont, Torrez-Espejo & Dujardin 1997 (Diptera: Psychodidae) belong to the pia series of the Lu. verrucarum species group, which includes several species that bite humans in Andean foci of leishmaniasis. The females of these two species exhibit isometry and isomorphism in anatomical structures of the head and terminalia commonly used in taxonomic identipcation of sand bies. They can only be differentiated based on subtle differences in the pigmentation of the pleura. In Lu. tihuiliensis, this is restricted to the basal portions of the katepimeron and katepisternum, whereas in Lu. piaboth structures are totally pigmented. Takinginto account the subtle morphological differences between these species, the objective of the current study was to evaluate the specipc taxonomic status of Lu. tihuiliensis with respect to Lu. pia. A 475-bp portion of the mitochondrial genome was sequenced, composed of the 3 end of the cytochrome b gene, intergenic spacer 1, the transfer RNA gene for serine, intergenic spacer 2, and the 3 end of the gene NAD dehydrogenase 1. Genetic analysis conÞrms that Lu. tihuiliensis and Lu. pia constitute two distinct species and this is supported by four strong lines of evidence, i.e., the paired genetic distances, size differences and amino acid composition of the cytochrome b protein, presence and absence of intergenic spacer one and divergence observed in the sequence of the transfer RNA gene for serine. It also conÞrms the validity of the pleural pigmentation pattern as a species diagnostic character and the importance of performing a detailed examination of this character during morphological determination of phlebotomine sand flies in the series pia.Publicación Acceso abierto Una nueva especie de Trichomyia Haliday (Diptera, Psychodidae) de Los Montes de María, Colombia.(Curitiba, Brasil: Revista Brasileira de Entomologia , 2010., 2019-10-10) Pérez Doria, Alveiro; Hernández, Ela; Bejarano, Eduar Elías; Sociedade Brasileira de Entomologia.A new species of Trichomyia Haliday (Diptera, Psychodidae) from Los Montes de María, Colombia. Trichomyia colosensis n. sp. is described and illustrated from male specimens collected with a modified CDC light trap in the Reserva Forestal Protectora Serranía de Coraza y Montes de María, on the Caribbean Coast of Colombia. To date, two species of Trichomyia have been reported from the Colombian Caribbean, T. brevitarsa (Rapp, 1945) and T. colosensis n. sp.Publicación Acceso abierto Nuevos registros de flebotomíneos (Diptera: Psychodidae), con el hallazgo de Lutzomyia longipalpis (Lutz & Neiva, 1912), en los alrededores de la Ciudad de Sincelejo, Colombia.(São Paulo, Brasil:Biota Neotropica, 2009., 2019-10-11) Vivero, Rafael José; Bejarano, Eduar Elías; Pérez Doria, Alveiro; Flórez, Fernando; Estrada, Luis Gregorio; Torres, Carolina; Muskus, CarlosThis is the first report of Lutzomyia longipalpis (Lutz & Neiva, 1912), L. shannoni (Dyar, 1929), L. micropyga (Mangabeira, 1942) and L. atroclavata (Knab, 1913) in the surroundings of an urban settlement in the Caribbean coast. Phlebotomine sandflies were collected by active search using suction devices and mouth aspirators in trees around the city of Sincelejo, the capital of the department of Sucre. Epidemiological importance derived from this finding is discussed due to the vectorial capacity of L. longipalpis, the natural vector of Leishmania infantum Nicolle, 1908 in America.Publicación Acceso abierto Predicción de la estructura secundaria del tRNASer (UCN) mitocondrial del flebotomíneo Lutzomyia hartmani (Diptera: Psychodidae).(Bogotá, Colombia: Acta Biológica Colombiana , 2011., 2019-10-09) Pérez Doria, Alveiro; Bejarano, Eduar E.Lutzomyia (Helcocyrtomyia) hartmanni is a sand fly that has been implicated in the transmission of Leishmania (Viannia) colombiensis, an etiologic agent of cutaneous leishmaniasis in Colombia. The objective of this work was to explore the potential usefulness of the mitochondrial serine transfer RNA (UCN) (tRNASer) in the taxonomic determination of L. hartmanni. Mitochondrial DNA was extracted, amplified and sequenced from entomological material collected in Envigado, Antioquia, Colombia. The tRNASer gene length was 68 nucleotide pairs, with an average adenine-thymine content of 80,9%. The studied tRNASer differs from other sand fly tRNASer known to date, on the basis of its primary and secondary structure. The observed number of intrachain base pairing was 7 in the acceptor arm, 3 in the dihydrouridine (DHU) arm, 5 in the anticodon arm, and 5 in the ribothymidine-pseudouridine-cytosine (T C) arm. The size of the DHU, anticodon, variable and T C loops was estimated to be 5, 7, 4, and 8 nucleotides, respectively. The notorious absence of non-Watson-Crick base pairs in the four arms of the tRNASer distinguishes that of L. hartmanni from others Lutzomyia spp.Publicación Acceso abierto Presencia de Lutzomyia dubitans (Sherlock, 1962) (Diptera: Psychodidae: Phlebotominae) en la Ciudad de Sincelejo, Departamento de Sucre, Colombia.(España :Boletín de la Asociación española de Entomología, 2006., 2019-11-07) Pérez Doria, Alveiro; Bejarano, Eduar Elías; Blanco Tuirán, Pedro José; Asociación española de Entomología.El objetivo de esta nota es informar del hallazgo de Lutzomyia dubitans (Sherlock, 1962), en zona urbana de la ciudad de Sincelejo, Departamento de Sucre, Colombia. Los flebotomíneos fueron colectados con trampas de luz tipo CDC instaladas en el peridomicilio. Este hallazgo eleva a ocho el número de especies de Lutzomyia registradas en la ciudad, lo que pone en evidencia la tendencia hacia la colonización de áreas urbanas por los insectos transmisores de leishmaniosis.Publicación Acceso abierto Primer hallazgo de Lutzomyia tihuiliensis (Diptera: Psychodidae) en el valle de Aburrá, Colombia.(Bogotá, Colombia: Revista Biomédica, 2006., 2019-10-24) Bejarano, Eduar Elías; Sierra, Diana; Pérez Doria, Alveiro; Darío Vélez., IvánThree of the seven species that comprise the pia series of the Lutzomyia verrucarum group have been recorded in Colombia, including L. pia, L. limafalcaoae and L. emberai. Objective. The aim of this paper is to report the occurrence of an anthropophilic morphospecies of the pia series in the country.