Examinando por Autor "Estrada, Luis Gregorio"
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Publicación Acceso abierto DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites.(Auckland, Nueva Zelanda: Revista Zootaxa, 2017., 2019-09-25) Vivero, Rafael José; Bejarano, Eduar Elías; Estrada, Luis Gregorio; Flórez, Fernando; Uribe Soto, Sandra; Yamileth Edgar; Estrada, Luis Gregorio; Ortega Gómez, Edgar; Torres Gutiérrez, Carolina; Muskus López, CarlosAlthough phlebotomine sand flies breeding sites have been identified and recorded by several studies, the microhabitats exploited by these insects remain little-known and hard to find. In this context, the difficulty of finding immature stages, and the limited number of taxonomic studies to identify immature stages of phlebotomine sand flies, are considered the major obstacles when attempting a complete inventory of Lutzomyia species. The objective of this study is to validate Cytochrome Oxidase I (Barcode region) as a marker for the identification of immature stages of Lutzomyia species recovered from natural breeding sites in Colombia. Among 142 collected sand flies, 18 immature individuals that did not complete their life cycle were identified to species level through sequencing of the COI gene. Values of K2P genetic distance between 0.002–0.031 allowed the identification of larvae at species level. The bootstrap support values (96%) in the Neighbor-Joining dendrogram were consistent for the majority of the established MOTUS of Lutzomyia atroclavata, Lutzomyia micropyga, Lutzomyia serrana, Lutzomyia cayennensis, Lutzomyia rangeliana, Lutzomyia shannoni and some species of the genus Brumptomyia. The COI gene is validated as a marker for the identification of immature stages of the genus Lutzomyia.Publicación Acceso abierto Nuevos registros de flebotomíneos (Diptera: Psychodidae), con el hallazgo de Lutzomyia longipalpis (Lutz & Neiva, 1912), en los alrededores de la Ciudad de Sincelejo, Colombia.(São Paulo, Brasil:Biota Neotropica, 2009., 2019-10-11) Vivero, Rafael José; Bejarano, Eduar Elías; Pérez Doria, Alveiro; Flórez, Fernando; Estrada, Luis Gregorio; Torres, Carolina; Muskus, CarlosThis is the first report of Lutzomyia longipalpis (Lutz & Neiva, 1912), L. shannoni (Dyar, 1929), L. micropyga (Mangabeira, 1942) and L. atroclavata (Knab, 1913) in the surroundings of an urban settlement in the Caribbean coast. Phlebotomine sandflies were collected by active search using suction devices and mouth aspirators in trees around the city of Sincelejo, the capital of the department of Sucre. Epidemiological importance derived from this finding is discussed due to the vectorial capacity of L. longipalpis, the natural vector of Leishmania infantum Nicolle, 1908 in America.Publicación Acceso abierto Registros nuevos de especies de Lutzomyia (Diptera: Psychodidae) en el departamento de Cesar, Colombia.(Bogotá, Colombia: Acta Biológica Colombiana , 2015., 2019-10-02) Estrada, Luis Gregorio; Aponte, Odethe Alejandra; Bejarano, Eduar ElíasStudies of the insects associated with the epidemiological cycle of leishmaniasis on the Caribbean coast of Colombia have been focused on the departments of Sucre and Córdoba. The phlebotomine sand fly fauna of the rest of the Caribbean region remains little known. In the present study four species of the genus Lutzomyia are reported from the department of Cesar for the first time. Sand flies were sampled using two CDC light traps with blue color LEDs at Balneario Hurtado, a tourist area in the city of Valledupar. A total of 50 phlebotomine sand flies were collected, of which the species Lutzomyia cayennensis cayennensis (38 % of the sample) and L. trinidadensis (14 %) were already known from the departament. Specimens of L. evansi (28 %), L. venezuelensis (10 %), L. micropyga (6 %) and L. rangeliana (2 %) represent the first records of these species for Cesar, raising the number of known occurrences from the department to eight species. Noteworthy among the new records is the sandfly L. evansi, recognized vector de Leishmania spp. in the Colombian Caribbean coast.