Examinando por Autor "Blanco, Pedro"
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Publicación Acceso abierto Evaluación de antígenos recombinantes SAG1 y GRA7 en el diagnóstico de la toxoplasmosis congénita, en el departamento de Sucre.(Bogotá, Colombia : Revista biomédica, 2015., 2019-10-01) Orozco, Kelly; Montero, Yina; Assia, Yulenis; Blanco, PedroLa toxoplasmosis congénita es el resultado de la afectación del feto como resultado de la infección primaria por Toxoplasma gondii en la mujer embarazada. Puesto que la infección por el parásito no presenta síntomas específicos, el diagnóstico preciso y rápido de la infección aguda en la mujer embarazada, es de vital importancia para ofrecer un tratamiento oportuno y reducir el riesgo de transmisión y la gravedad de la enfermedad. Las limitaciones de las técnicas serológicas utilizadas para el diagnóstico de la toxoplasmosis, hacen necesario validar nuevas metodologías que permitan aumentar su sensibilidad y especificidad, con el fin de obtener una prueba que brinde resultados muy confiables y un diagnóstico correcto. El objetivo de esta investigación, es valuar los antígenos recombinantes SAG1 y GRA7 en la detección de anticuerpos IgG anti-Toxoplasma, en mujeres gestantes del departamento de Sucre.Publicación Acceso abierto Genetic Characterization of Northwestern Colombian Chikungunya Virus Strains from the 2014–2015 Epidemic.(Estados Unidos: The American Journal of Tropical Medicine and Hygiene , 2016., 2019-09-27) Rodas, Juan D.; Kautz, Tiffany; Camacho, Erwin; Paternina, Luis; Díaz, Francisco J.; Tesh, Robert; Weaver, Scott C.; Blanco, Pedro; Guzmán, Hilda; The American Society of Tropical Medicine and Hygiene.Chikungunya fever, an acute and often chronic arthralgic disease caused by the mosquito-borne alphavirus,chikungunya virus (CHIKV), spread into the Americas in late 2013. Since then it has caused epidemics in nearly all New World countries, the second largest being Colombia with over 450,000 suspected cases beginning in September, 2014, and focused in Bolivar Department in the north. We examined 32 human sera from suspected cases, including diverse age groups and both genders, and sequenced the CHIKV envelope glycoprotein genes, known determinants of vector host range. As expected for Asian lineage CHIKV strains, these isolates lacked known Aedes albopictus–adaptive mutations. All the Colombian strains were closely related to those from the Virgin Islands, Saint Lucia, Mexico, Puerto Rico, and Brazil, consistent with a single, point-source introduction from the southeast Asia/Pacific region. Two substitutions in the E2 and E1 envelope glycoprotein genes were found in the Colombian strains, especially E1-K211E involving a residue shown previously to affect epistatically the penetrance of the E1-A226V A. albopictus–adaptive substitution. We also identified two amino acid substitutions unique to all American CHIKV sequences: E2-V368A and 6K-L20M. Only one codon, 6K-47, had a high nonsynonymous substitution rate suggesting positive selection.Publicación Acceso abierto Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series.(Estados Unidos, American Journal of Tropical Medicine and Hygiene , 2015., 2019-10-30) Gómez Carmago, Doris E.; Blanco, Pedro; Mora García, Gustavo; Paternina, Margareth; Caraballo, Libardo; American Society of Tropical Medicine and Hygiene.Leptospirosis is a public health problem on the Colombian Caribbean Coast. In Cartagena, a major city in this region, this disease is endemic with high annual lethality. In clinical practice, differential diagnosis of leptospirosis with others icteric febrile syndromes is crucial to an appropriated therapeutic approach. Nevertheless, high complexity hospitals employ only serological test to confirm suspicious cases, with an elevated frequency of undetermined results. Therefore, the aim of this study was to apply molecular techniques in patients under leptospirosis suspicion. It was carried out a case series study with adults in 2013 last trimester. Patients with clinical manifestations and positive/undetermined results in serological test for leptospirosis were included. We used Polymerase Chain Reaction to detect Leptospira spp from blood samples with specific primers that amplify a DNA segment from the lipl32 gen. A total of 9 patients were included (3 women, 6 men). IgM test for leptospirosis was positive in 5 subjects and undetermined in 4 others. A 423pb product was expected, however only 3 samples showed an amplicon of 358pb. When those DNA fragments were sequenced, none of them were part of Leptospira spp. genome. Thereafter, samples were amplified for Riskettsia spp. by nested PCR for the OmpB gene. Seven samples were positive (420pb amplicon). These samples were also positive for Rickettsia spp 17KDa gene (230pb amplicon).Publicación Acceso abierto Seroprevalencia de la enfermedad de Chagas y factores de riesgo asociados en una población de Morroa, Sucre.(Bogotá, Colombia: Revista Biomédica, 2007., 2019-10-22) Hoyos, Richard; Pacheco, Lisandro; Agudelo, Luz Adriana; Zafra, German; Blanco, Pedro; Triana, OmarChagas disease is a major public health problem in Latin America. In Colombia, a large area has the ecoepidemiological conditions which favor the active transmition of this infection. This study was undertaken in a population from the municipality of Morroa, Sucre Province, to evaluate risk factors and to determine the seroprevalence to Chagas disease.Publicación Acceso abierto Seroprevalencia de infección por Toxoplasma gondii en mujeres gestantes de Sucre.(Bogotá, Colombia : Revista biomédica, 2011., 2019-10-08) Assia, Yulenis; Montero, Yina; Orozco , Kelly; Blanco, PedroLa toxoplasmosis es una infección producida por el protozoo Toxoplasma gondii. La importancia de la toxoplasmosis reside, principalmente, en la forma congénita y la reactivación en el inmunodeprimido. Cuando la infección es adquirida por primera vez durante el embarazo, puede producir toxoplasmosis congénita, que se presenta en el recién nacido con diversas manifestaciones clínicas, como hidrocefalia, microcefalia y coriorretinitis, o terminar en un aborto. En mujeres gestantes del departamento de Sucre, se ha reportado una alta tasa de seroconversión de infección por T. gondii. Sin embargo, la vigilancia serológica aún no se ha incluido en el control prenatal en embarazadas. El objetivo del presente estudio fue determinar la seroprevalencia de infección por T. gondii en gestantes del departamento de Sucre.Publicación Acceso abierto Successful sporozoite challenge model in human volunteers with plasmodium vivax strain derived from human donors.(Estados Unidos: American Journal of Tropical Medicine and Hygiene, 2009., 2019-10-11) Herrera, Sócrates; Fernández, Olga; Manzano, María R.; Murrain, Bermans; Vergara, Juana; Blanco, Pedro; Palacios, Ricardo; Vélez, Juan D.; Epstein, Judith E.; Chen-Mok, Mario; Reed, ZarifahH; Arévalo Herrera, Myriam; American Society of Tropical Medicine and Hygiene.Successful establishment of a Plasmodium vivax sporozoite challenge model in humans isdescribed. Eighteen healthy adult, malaria-naïve volunteers were randomly allocated to Groups A–C and exposed to 3 ± 1, 6 ± 1, and 9 ± 1 bites of Anopheles albimanus mosquitoes infected with P.vivax, respectively. Seventeen volunteers developed signs and symptoms consistent with malaria,and geometric mean prepatent periods of 11.1 days (9.3–11) for Group A; 10.8 days (9.8–11.9) forGroup B; and 10.6 days (8.7–12.4) for Group C, with no statistically significant difference amonggroups (Kruskal-Wallis, P = 0.70). One volunteer exposed to eight mosquito bites did not developa parasitemia. No differences in parasite density were observed and all individuals successfullyrecovered after anti-malarial treatment. None of the volunteers developed parasite relapses withinan 18-month follow-up. In conclusion, malaria-naive volunteers can be safely and reproducibly infected with bites of 2–10 An. albimanus mosquitoes carrying P. vivax sporozoites. Thischallenge method is suitable for vaccine and anti-malarial drug testing.