Examinando por Autor "Vivero, Rafael José"
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Publicación Acceso abierto Análisis de la estructura primaria y secundaria del ARN de transferencia mitocondrial para serina en siete especies de Lutzomyia.(Bogotá, Colombia: Revista Biomédica, 2007., 2019-10-21) Vivero, Rafael José; Contreras Gutiérrez, María Angélica; Bejarano, Eduar ElíasLutzomyia sand flies are involved in the transmission of the parasite Leishmania spp. in America. The taxonomy of these vectors is traditionally based on morphological features of the adult stage, particularly the paired structures of the head and genitalia. Although these characters are useful to distinguish most species of Lutzomyia, morphological identification may be complicated by the similarities within subgenera and species group. To evaluate the utility of mitochondrial serine transfer RNA tRNASer for taxonomic identification of Lutzomyia.Publicación Acceso abierto Cambios en el extremo carboxilo terminal de citocromo b como carácter taxonómico en Lutzomyia (Diptera: Psychodidae).(Bogotá, Colombia : Revista Colombiana de Entomología, 2009., 2019-10-16) Vivero, Rafael José; Contreras Gutiérrez, María Angélica; Bejarano, Eduar Elías; Sociedad Colombiana de Entomología .Morphological characters have been traditionally used for identification of sand flies of the genus Lutzomyia; however, their utility is limited in some species. In this work, we characterized the mitochondrial DNA region coding for the carboxyl-terminal domain of cytochrome b protein in seven species of Lutzomyia: L. trinidadensis, L. panamensis, L. cayennensis cayennensis, L. dubitans, L. gomezi, L. rangeliana and L. evansi. A total of 134 polymorphic sites were detected in the gene (40.98%) and 29 sites in the protein (26.6%). The very high level of polymorphism observed in cytochrome b, included replacement of amino acids, use of alternative stop codons, and differences in the size of the protein. The utility of the studied region in the identification of Lutzomyia species is discussed.Publicación Acceso abierto Description of Lutzomyia velezi, a new species of phlebotomine sand fly (Diptera: Psychodidae) from the Department of Antioquia, Colombia.(Rio de Janeiro, Brazil: Memórias do Instituto Oswaldo Cruz, 2010., 2019-10-10) Bejarano, Eduar Elías; Vivero, Rafael José; Uribe, SandraThe phlebotomine sand fly Lutzomyia velezi sp.nov. was described and illustrated from male specimens collected by light trap in the Reserva Natural Cañon del Río Claro in the Central Cordillera of the Colombian Andes. The new species belongs to the series sanguinaria of the subgenus Helcocyrtomyia, which is represented in Colombia by Lutzomyia cirrita, Lutzomyia hartmanni, Lutzomyia sanguinaria, Lutzomyia scorzai, Lutzomyia sp. of Pichindé and Lutzomyia tortura. The new species can be differentiated from others of the subgenus by the combination of the fol- lowing characteristics: long antennal ascoids, reaching level of the papilla, coxite with a single basal seta and fifth palpomere longer than or equal to the sum of the lengths of the third and fourth palpomeres.Publicación Acceso abierto DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites.(Auckland, Nueva Zelanda: Revista Zootaxa, 2017., 2019-09-25) Vivero, Rafael José; Bejarano, Eduar Elías; Estrada, Luis Gregorio; Flórez, Fernando; Uribe Soto, Sandra; Yamileth Edgar; Estrada, Luis Gregorio; Ortega Gómez, Edgar; Torres Gutiérrez, Carolina; Muskus López, CarlosAlthough phlebotomine sand flies breeding sites have been identified and recorded by several studies, the microhabitats exploited by these insects remain little-known and hard to find. In this context, the difficulty of finding immature stages, and the limited number of taxonomic studies to identify immature stages of phlebotomine sand flies, are considered the major obstacles when attempting a complete inventory of Lutzomyia species. The objective of this study is to validate Cytochrome Oxidase I (Barcode region) as a marker for the identification of immature stages of Lutzomyia species recovered from natural breeding sites in Colombia. Among 142 collected sand flies, 18 immature individuals that did not complete their life cycle were identified to species level through sequencing of the COI gene. Values of K2P genetic distance between 0.002–0.031 allowed the identification of larvae at species level. The bootstrap support values (96%) in the Neighbor-Joining dendrogram were consistent for the majority of the established MOTUS of Lutzomyia atroclavata, Lutzomyia micropyga, Lutzomyia serrana, Lutzomyia cayennensis, Lutzomyia rangeliana, Lutzomyia shannoni and some species of the genus Brumptomyia. The COI gene is validated as a marker for the identification of immature stages of the genus Lutzomyia.Publicación Acceso abierto Nuevos registros de flebotomíneos (Diptera: Psychodidae) en el área de influencia del río Amoyá en Chaparral, Tolima.(Bogotá, Colombia: Revista Biomédica, 2012., 2019-10-04) Contreras, María Angélica; Vivero, Rafael José; Bejarano, Eduar Elías; Carrillo, Lina María; Carrillo, Lina María; Vélez, Ivan DaríoIn Colombia, the diversity of phlebotomine sand flies is high, with 162 recorded species, and which include vectors of Leishmania spp. The objective of this research is to identify the the sand fly species of medically importance in the area of influence from Amoyá River Hydroelectric Project, Colombia.Publicación Acceso abierto Nuevos registros de flebotomíneos (Diptera: Psychodidae), con el hallazgo de Lutzomyia longipalpis (Lutz & Neiva, 1912), en los alrededores de la Ciudad de Sincelejo, Colombia.(São Paulo, Brasil:Biota Neotropica, 2009., 2019-10-11) Vivero, Rafael José; Bejarano, Eduar Elías; Pérez Doria, Alveiro; Flórez, Fernando; Estrada, Luis Gregorio; Torres, Carolina; Muskus, CarlosThis is the first report of Lutzomyia longipalpis (Lutz & Neiva, 1912), L. shannoni (Dyar, 1929), L. micropyga (Mangabeira, 1942) and L. atroclavata (Knab, 1913) in the surroundings of an urban settlement in the Caribbean coast. Phlebotomine sandflies were collected by active search using suction devices and mouth aspirators in trees around the city of Sincelejo, the capital of the department of Sucre. Epidemiological importance derived from this finding is discussed due to the vectorial capacity of L. longipalpis, the natural vector of Leishmania infantum Nicolle, 1908 in America.Publicación Acceso abierto Primer registro del flebotomíneo Warileya (Hertigia) hertigi en Antioquia, Colombia.(Bogotá, Colombia: Revista Biomédica, 2018., 2019-09-20) Bejarano, Eduar Elías; Uribe, Sandra; Vivero, Rafael JoséThe genus Warileya is one of the least-known taxa of sandflies, comprising only nine species, i.e., W. (Warileya) phlebotomanica, W. (Hertigia) hertigi, W. (W.) rotundipennis, W. (W.) nigrosacculus, W. (W.) yungasi, W. (W.) fourgassiensis, W. (W.) lumbrerasi, W. (W.) euniceae and W. (W.) leponti.Publicación Acceso abierto Trece registros nuevos de Lutzomyia (Diptera: Psychodidae) para el departamento de Vichada, Orinoquia Colombiana.(São Paulo, Brasil : Biota Neotropica, 2010., 2019-10-11) Vivero, Rafael José; Bejarano, Eduar Elías; Castro, Mirley; Vélez, Andrés; Pérez, Juan Esteban; Pérez Doria, Alveiro; Vélez, Iván DaríoLutzomyia trinidadensis (Newstead, 1922), L. bettinii Feliciangeli, Ramírez-Pérez & Ramírez, 1988, L. flaviscutellata (Mangabeira, 1942), L. yuilli Young & Porter, 1972, L. saulensis (Floch & Abonnenc, 1944), L. runoides (Fairchild & Hertig, 1953), L. ayrozai (Barretto & Coutinho, 1940), L. claustrei Abonnenc, Leger & Fauran, 1979, L. walkeri (Newstead, 1914), L. preclara Young & Arias, 1984, L. (geniculata) sp., L. (squamiventris) sp., and L. (Evandromyia) sp., are recorded for the first time in the department of Vichada, Colombia. In addition, the presence of L. antunesi (Coutinho, 1939) and L. aragaoi (Costa Lima, 1932) in this department is confirmed. Sand flies were collected in the municipalities of Puerto Carreño, Cumaribo, and La Primavera, using CDC light traps, Shannon trap, and human bait, and by active search in resting places. The epidemiological importance of these sand fly species is discussed in relation with the cutaneous leishmaniasis.